Fluo-3 ammonium salt | CAS 339221-91-9

Catalog Number	A14-0031
Category	Calcium, Chloride and Other indicators
Molecular Formula	C36H25Cl2N2O13·5NH4
Molecular Weight	854.7

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Product Introduction

Fluo-3 is a fluorescent indicator for intracellular Ca2+ that is used in flow cytometry and cell-based experiments. Fluo-3 produces fluorescence with an emission maximum at 525 nm upon binding to calcium.

Chemical Information
Product Specification
Application

Related CAS	123632-39-3 (free acid)
Synonyms	N-[2-[2-[2-[bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylphenyl]-N-(carboxymethyl)-glycine, pentaammonium salt
Purity	≥90%
Canonical SMILES	CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)[O-])Cl)N(CC(=O)[O-])CC(=O)[O-].[NH4+].[NH4+].[NH4+].[NH4+].[NH4+]
InChI	InChI=1S/C36H30Cl2N2O13.5H3N/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36;;;;;/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50);5*1H3
InChIKey	ABPVTPOQYAKASW-UHFFFAOYSA-N
Appearance	Solid Powder

Excitation	488 nm
Emission	526 nm
Storage	Store at -20°C

Fluo-3 ammonium salt, a high-affinity calcium indicator, plays a crucial role in biological research. Here are four key applications of Fluo-3 ammonium salt:

Intracellular Calcium Imaging: Delving into the realm of cellular dynamics, Fluo-3 ammonium salt serves as a powerful tool for monitoring calcium fluctuations within cells. As cells laden with Fluo-3 encounter calcium influx, the dye emits a fluorescence signal, enabling researchers to visually perceive calcium transients. This capability is vital for dissecting intricate cellular processes like signal transduction, muscle contraction, and neurotransmitter release.

Drug Discovery and Screening: At the forefront of drug development, Fluo-3 ammonium salt takes center stage in high-throughput screening assays aimed at assessing compound effects on intracellular calcium levels. By detecting alterations in fluorescence intensity, scientists can pinpoint potential drug candidates that modulate calcium signaling pathways. This methodology holds significant promise for unearthing novel therapeutics for conditions such as cardiac arrhythmias and neurodegenerative disorders.

Neuroscience Research: Navigating the complexities of the brain, Fluo-3 ammonium salt proves its worth in investigating calcium signaling dynamics within neurons and other neural tissues. Employing calcium imaging with Fluo-3 sheds light on synaptic activity, neural network behaviors, and mechanisms underlying learning and memory formation. This indispensable tool propels our understanding of brain function and sheds light on the pathophysiology of neurological disorders.

Cell Physiology Studies: In the realm of cell biology, Fluo-3 ammonium salt emerges as a key ally in exploring calcium-dependent processes across diverse cell types. Researchers harness this tool to trace calcium oscillations, quantify local calcium concentrations, and decipher how calcium fluxes influence cellular functions such as secretion, growth, and cell death. This investigative approach unveils intricate nuances of cell physiology and provides insights into disease mechanisms at a cellular level.