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6-Carboxyfluorescein diacetate succinimidyl ester | CAS 150206-15-8
| Catalog Number | A16-0081 |
| Category | Cell Proliferation Tracer Fluorescent Probes |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.46 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
6-Carboxyfluorescein diacetate succinimidyl ester is an amine-reactive probe for labeling human intervertebral disk cells in vitro by fluorescence microscopy. It is also used for determining intracellular pH in bacteria by fluorometry.
Chemical Information
Product Specification
Application
| Synonyms | 6-CFDA N-succinimidyl ester; 6-CFDA SE; 6-Carboxy-fluorescein diacetate N-succinimidyl ester; (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate; 6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester; Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester; 2,5-Dioxo-1-pyrrolidinyl 3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylate; 2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-6-yl]carbonyl]oxy]- |
| Purity | ≥95% |
| IUPAC Name | (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate |
| Canonical SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)ON6C(=O)CCC6=O)C(=O)O3 |
| InChI | InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3 |
| InChIKey | JGPOSNWWINVNFV-UHFFFAOYSA-N |
| Solubility | Soluble in DMF |
| Density | 1.60±0.1 g/cm3 |
| Boiling Point | 765.4±70.0°C at 760 mmHg |
| Excitation | 553 |
| Emission | 575 |
| Storage | Store at -20°C |
Cell Proliferation Monitoring: 6-Carboxyfluorescein diacetate succinimidyl ester (CFDA SE) is widely used as a fluorescent probe for monitoring cell proliferation. Once inside the cell, it is cleaved by intracellular esterases, resulting in a fluorescent compound that can be retained in the cytoplasm. This property makes CFDA SE an excellent tool for tracking cell division over time, as the intensity of fluorescence increases with each round of cell division. Researchers utilize this probe to assess the effects of various treatments, such as drugs or growth factors, on cell proliferation. This application is especially relevant in cancer research, regenerative medicine, and developmental biology, where understanding cell growth dynamics is crucial for developing therapeutic strategies.
Cellular Tracking in Live Imaging: CFDA SE is also employed in live-cell imaging to track individual cell populations over time. Its ability to provide stable and bright fluorescence enables researchers to visualize cellular movements and interactions in real-time. This application is particularly useful in studying cell migration, differentiation, and tissue formation. By labeling specific cell types with CFDA SE, scientists can gain insights into how cells respond to their environment and how they communicate with neighboring cells. This capability is vital for understanding complex biological processes, such as wound healing and immune responses, providing a clearer picture of cellular behavior in vivo.
Evaluation of Drug Effects: In pharmacology, 6-Carboxyfluorescein diacetate succinimidyl ester is used to evaluate the effects of various compounds on cell viability and proliferation. Researchers can apply this probe in high-throughput screening assays to quickly assess the cytotoxic effects of potential drug candidates. By measuring the fluorescence intensity, scientists can determine the number of viable cells and how they respond to specific treatments. This application is crucial for drug development, allowing researchers to identify effective compounds while minimizing the time and resources needed for preliminary screening. The insights gained from these studies contribute to the optimization of drug formulations and therapeutic strategies.
Investigating Apoptosis and Cellular Stress: 6-Carboxyfluorescein diacetate succinimidyl ester is also valuable in studies investigating apoptosis and cellular stress responses. By analyzing changes in fluorescence intensity, researchers can infer whether cells are undergoing programmed cell death or experiencing stress conditions that affect their proliferation. This application helps in understanding the mechanisms of cell survival and death in response to various stimuli, including oxidative stress, nutrient deprivation, or exposure to toxic compounds. By linking fluorescence data with other assays, scientists can elucidate the pathways involved in cellular responses to stress and identify potential therapeutic targets for conditions such as cancer or neurodegenerative diseases.
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