5(6)-Carboxyfluorescein

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5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein | 72088-94-9

Catelog Number A15-0005
Category pH Indicators
Molecular Formula C21H12O7
Molecular Weight 376.3
Catalog Number Size Price Quantity
A15-0005 10 g $469
A15-0005 25 g $819

Product Introduction

5(6)-Carboxyfluorescein is a mixture of 5-carboxyfluorescein and 6-carboxyfluorescein. It is commonly used for measuring changes of intracellular pH.

Chemical Information

Synonyms 5(6)-FAM; 3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid
Purity ≥90%
IUPAC Name 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid;3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
Canonical SMILES C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O.C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChI InChI=1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)
InChI Key BPVHBBXCESDRKW-UHFFFAOYSA-N
Appearance Dark Orange to Brown Solid
Melting Point >250˚C (dec.)

Product Specification

Excitation 492 nm
Emission 514 nm
Storage Store at -20°C
Signal Warning
GHS Hazard Statements H317 (33.33%): May cause an allergic skin reaction [Warning Sensitization, Skin] H319 (66.67%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
Precautionary Statement Codes P261, P264+P265, P272, P280, P302+P352, P305+P351+P338, P321, P333+P313, P337+P317, P362+P364, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.)

Computed Properties

Hydrogen Bond Donor Count 6
Hydrogen Bond Acceptor Count 14
Rotatable Bond Count 2
Exact Mass 752.11660544 g/mol
Monoisotopic Mass 752.11660544 g/mol
Topological Polar Surface Area 227Ų
Heavy Atom Count 56
Formal Charge 0
Complexity 1270
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 2
Compound Is Canonicalized Yes

Patents

Publication NumberTitlePriority Date
WO-2021226036-A1 Compositions and methods for passive optical barcoding for multiplexed assays 2020-05-04
WO-2021150838-A1 Compositions and methods for cell-like calibration particles 2020-01-24
US-10481068-B2 Hydrogel particles with tunable optical properties 2012-04-06
US-2018252630-A1 Hydrogel particles with tunable optical properties 2012-04-06
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