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Indo-1 potassium salt | 132319-56-3
| Catalog Number | A14-0021 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C32H26K5N3O12 |
| Molecular Weight | 840.1 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0021 | -- | $-- |
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Product Introduction
Indo-1 is a ratiometric fluorescent indicator for calcium. Indo-1 is useful for flow cytometry. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.
Chemical Information |
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|---|---|
| Related CAS | 96314-96-4 (free acid) |
| Synonyms | Indo 1 Pentapotassium Salt; 2-[4-[bis(carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid, pentapotassium salt |
| Purity | ≥90% |
| Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+].[K+] |
| InChI | InChI=1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5 |
| InChI Key | VGZPRMSXVNDMAN-UHFFFAOYSA-I |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 349-364 nm |
| Emission | 475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound) |
| Storage | Store at -20°C |
Indo-1 potassium salt is a fluorescent calcium indicator dye widely used in biological research. Here are some key applications of Indo-1 potassium salt:
Cellular Calcium Imaging: Indo-1 potassium salt is extensively used to measure intracellular calcium levels in live cells. By binding to calcium ions, it emits fluorescence that can be detected using fluorescence microscopy or flow cytometry. This allows researchers to study calcium signaling pathways, explore cellular responses to stimuli, and investigate the effects of drugs on calcium dynamics.
Cardiovascular Research: In cardiovascular studies, Indo-1 potassium salt is used to monitor calcium transients in cardiac tissue. Calcium plays a pivotal role in the contraction and relaxation of heart muscles, and this dye helps in visualizing these processes in real time. It is instrumental in studying cardiac arrhythmias, heart failure, and the pharmacological effects of cardiotropic drugs.
Neuroscience: Indo-1 potassium salt is used in neuroscience to explore calcium-mediated neuronal activities. By monitoring cellular calcium changes, researchers can study neuron excitability, synaptic function, and neuroplasticity. This application is crucial for understanding brain function, disease mechanisms, and the development of neurotherapeutics.
Drug Screening: Indo-1 potassium salt is used in high-throughput drug screening assays to evaluate the effect of compounds on cellular calcium levels. By detecting changes in fluorescence, researchers can rapidly assess the activity of potential drug candidates that target calcium channels, transporters, or signaling pathways. This is valuable for the discovery and development of new therapeutic agents.
Applications of Fluorescent Probes & Dyes
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