1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea | CAS 957493-98-0

1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea is a spirocyclic xanthene-based fluorescent dye designed to provide strong fluorescence output upon appropriate activation, making it useful in fluorescence labeling and probe development workflows where low background is important. Its tertiary amine functionality supports conjugation and formulation strategies commonly used for constructing imaging reagents, while the xanthene core enables bright visible fluorescence compatible with standard fluorescence microscopy and plate-reader formats.

1. Fluorescent Labeling Conjugates

1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea is used as a dye building block for preparing fluorescent labeling conjugates for biomolecule tracking, including labeling of peptides, proteins, and other research reagents used in chemical biology. Researchers incorporate the dye into conjugation schemes to generate imaging-ready constructs for localization studies in cell-based assays, where the spirocyclic xanthene scaffold helps manage background fluorescence in labeling workflows. The resulting conjugates are commonly evaluated in fluorescence microscopy and fluorescence-based readouts to verify labeling performance and signal behavior in the relevant buffer and instrument settings.

1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea supports fluorescence imaging reagent development for microscopy applications that require a compact, high-contrast dye format. In practice, the dye is incorporated into targeted or non-targeted staining reagents for visualizing labeled biomolecular assemblies and tracking distribution in fixed or prepared samples, including samples used in mechanistic studies of biomolecular interactions. Its xanthene photophysics are leveraged to produce clear emission under common excitation/emission filter sets used in routine fluorescence microscopy, enabling straightforward integration into established imaging pipelines.

1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea is applied in fluorescence-based bioassay development where a dye with robust visible emission is needed as a reporter in assay formats using labeled reagents. Assay developers use the dye to construct fluorescent readouts for monitoring binding events, reagent uptake, or labeling-dependent signal changes in microplate and cuvette-based measurements. The dye's amine-containing structure supports downstream conjugate preparation strategies for assembling assay reagents, and its fluorescence output is used to generate quantifiable signals under standard fluorescence detection instrumentation.

1. Fluorescent Standards And Controls

1-(3',6'-bis(ethylamino)-2',7'-dimethyl-3-oxospiro[isoindoline-1,9'-xanthene]-2-yl)-3-phenylurea is also used to prepare fluorescence standards and assay controls for method development and instrument verification. Researchers and assay teams incorporate the dye into control materials to benchmark signal response across experiments, including comparisons across different labeling batches, imaging sessions, or plate-reader runs. By providing a consistent fluorescent reference material, the dye helps teams normalize fluorescence measurements and validate that assay readouts remain stable under the specific experimental conditions used for their fluorescent labeling and imaging studies.