Indo 1 | CAS 96314-96-4

Catalog Number	A21-0018
Category	Calcium, Chloride and Other indicators
Molecular Formula	C32H26K5N3O12
Molecular Weight	840.05

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Product Introduction

Indo 1 is a fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium.

Chemical Information
Product Specification
Application

Synonyms	4-(6-CARBOXY-2-INDOLYL)-4'-METHYL-2,2-(ETHYLENEDIOXY)DIANILINE-N,N,N',N'-TETRAACETIC ACID PENTAPOTASSIUM SALT; INDO 1 PENTAPOTASSIUM SALT; INDO 1; INDO 1, FOR FLUORESCENCE; INDO 1 PENTASODIUM SALT >95% FLUORESCENT MARKER FO; 4-(6-carboxy-2-indolyl)-4'-methyl
Purity	95%
IUPAC Name	2-[4-[bis(carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid
Canonical SMILES	CC1=CC(=C(C=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)O)N(CC(=O)O)CC(=O)O
InChI	InChI=1S/C32H31N3O12/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45)
InChIKey	AMHAQOBUZCQMHN-UHFFFAOYSA-N
Density	1.527g/cm3
Appearance	Solid
Boiling Point	1016.3°C at 760mmHg

Storage

-20°C

Indo 1, a fluorescent calcium indicator, plays a vital role in bioscience for measuring intracellular calcium levels. Here are four key applications of Indo 1:

Calcium Signaling Studies: Renowned for its exceptional sensitivity to calcium, Indo 1 serves as an indispensable tool for delving into calcium signaling dynamics across diverse cell types. Researchers harness this indicator to track real-time fluctuations in intracellular calcium concentrations, shedding light on essential cellular functions like muscle contraction, neurotransmitter release, and hormone secretion. This deep dive unveils the intricate mechanisms governing calcium-dependent signaling pathways, enriching our comprehension of cellular communication.

Cardiovascular Research: Within the realm of cardiovascular research, Indo 1 takes center stage in unraveling the intricacies of calcium dynamics within cardiac cells. The orchestration of calcium levels is paramount to the rhythmic dance of heart muscle contraction and relaxation, with deviations signaling potential cardiac disorders. By monitoring intracellular calcium transients with precision using Indo 1, scientists navigate the terrain of heart physiology and pathophysiology, paving the way for innovative treatments targeting conditions such as arrhythmias and heart failure.

Neurophysiology: In the realm of neurophysiology, Indo 1 emerges as a key player in exploring calcium influx and homeostasis within neurons. Calcium ions wield significant influence over various neuronal functions, including synaptic plasticity, memory formation, and neurotransmitter release. Leveraging Indo 1, researchers gain a visual and quantitative lens into calcium dynamics within neurons, enriching the study of brain function and neurological disorders. This nuanced investigation fuels our understanding of the intricate mechanisms underpinning neural communication.

Drug Screening: Indo 1 transcends its role as a calcium indicator to shine in high-throughput screening assays evaluating the impact of potential pharmacological agents on calcium signaling pathways. By capturing changes in intracellular calcium levels, researchers unveil the efficacy and toxicity profiles of novel drugs on calcium-dependent processes. This exploration facilitates the discovery and development of groundbreaking therapeutics targeting calcium channels and receptors, opening new avenues for drug innovation and precision medicine.