Trisulfo-Cy5-Alkyne | CAS 2055138-90-2

Trisulfo-Cy5-Alkyne is a Cy5-based fluorescent labeling building block bearing a terminal alkyne for click chemistry conjugation, along with sulfonate groups that support aqueous handling and fluorescence readouts in biological assay workflows. This alkyne handle enables efficient installation of Cy5 into biomolecules, targeting ligands, and imaging reagents via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or related click formats, supporting downstream fluorescence microscopy, flow cytometry, and fluorescence-based characterization of conjugates.

1. Biomolecule Click Labeling

Trisulfo-Cy5-Alkyne is routinely used by chemical biology and bioconjugation teams to install Cy5 fluorophores onto azide-functionalized proteins, peptides, and affinity reagents for fluorescence tracking and reagent characterization. The terminal alkyne provides a defined attachment point that helps maintain consistent labeling chemistry across batches, while the sulfonated Cy5 scaffold supports aqueous conjugate preparation for labeling workflows used in assay development and imaging reagent qualification.

2. Fluorescence Microscopy Tracing

Trisulfo-Cy5-Alkyne supports fluorescence microscopy studies where researchers need a robust Cy5 signal for visualizing labeled biomolecular assemblies, surface-bound ligands, or fluorescently tagged materials. After click conjugation to an azide-bearing targeting moiety or biomaterial, the resulting Cy5 conjugates are used to monitor localization patterns, wash behavior, and staining retention in microscopy experiments that rely on far-red fluorescence channels.

3. Flow Cytometry Conjugate Building

Trisulfo-Cy5-Alkyne is used in flow cytometry dye-conjugate development to generate far-red fluorescent conjugates from azide-functional biomolecules, enabling multicolor panel construction and reagent benchmarking. In typical workflows, the click-installed Cy5 label provides a stable fluorescent tag for quantifying binding or association of labeled constructs with cells or cell-derived targets, supporting assay optimization and experimental reproducibility in fluorescence-activated workflows.

4. Surface and Material Functionalization

Trisulfo-Cy5-Alkyne is also applied in biomaterials and surface engineering projects where azide-functional surfaces, hydrogels, or polymer coatings are functionalized with Cy5 for fluorescence readouts. By using the alkyne handle to create covalently attached fluorescent coatings, researchers can evaluate surface coverage, spatial distribution, and post-functionalization performance in imaging and material characterization experiments that benefit from far-red fluorescence detection.