Sulfo-Cyanine5 amine | 2183440-44-8

Sulfo-Cyanine5 amine, a far-red fluorescent dye widely utilized in various bioimaging and labeling applications, offers a multitude of key applications with elevated levels of perplexity and burstiness:

Fluorescence Microscopy: Leveraged in fluorescence microscopy, Sulfo-Cyanine5 amine serves as a vital tool for visualizing cellular structures and molecular interactions. The far-red fluorescence not only enhances imaging depth but also diminishes background noise, resulting in clearer and more intricate images. Its utility is particularly evident in multi-color imaging experiments, where its unique emission wavelength stands out distinctly among other fluorophores.

Flow Cytometry: In the realm of flow cytometry, Sulfo-Cyanine5 amine plays a pivotal role in labeling antibodies and other biomolecules for cell sorting and analysis. With its robust and consistent fluorescence, it enables the detection and quantification of low-abundance targets within complex cell populations, facilitating precise phenotyping and functional analysis of cells in both research and clinical diagnostics.

Fluorescence Resonance Energy Transfer (FRET): Positioned at the forefront of FRET experiments, Sulfo-Cyanine5 amine is instrumental in studying protein-protein and protein-DNA interactions. Whether acting as a donor or acceptor molecule, it offers valuable insights into molecular proximity and interaction dynamics, crucial for unraveling complex biological processes at a molecular level.

Western Blotting: Within the domain of Western blotting, Sulfo-Cyanine5 amine serves as a fluorescent probe for detecting specific proteins. By conjugating the dye to secondary antibodies, researchers can visualize and quantify protein bands with exceptional sensitivity. This method’s versatility allows for multiplexing, enabling the simultaneous detection of multiple targets on a single blot, advancing the capabilities of protein analysis in a dynamic and comprehensive manner.