Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 | CAS 2353410-10-1

Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 is a sulfonated, water-soluble Cy5-family fluorescent dye designed for robust fluorescent labeling workflows where aqueous compatibility and strong far-red emission are advantageous. Its carboxylate functionality supports conjugation strategies to biomolecules and surfaces, enabling fluorescent tracking and imaging readouts in microscopy and fluorescence-based assays. Researchers commonly employ this dye to generate Cy5-labeled conjugates for sensitive visualization across standard fluorescence instrumentation.

1. Protein And Peptide Labeling

Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 is used to prepare fluorescent protein and peptide conjugates for cellular imaging, binding studies, and fluorescence-based characterization assays. In typical workflows, the dye is incorporated into labeling schemes that yield far-red fluorescent conjugates compatible with aqueous buffers, supporting downstream sample handling for microscopy and plate-based readouts. Researchers in chemical biology and bioconjugation chemistry often choose sulfonated Cy5 derivatives to maintain fluorescence in water-rich environments while providing a bright far-red signal for tracking biomolecules in complex mixtures.

2. Fluorescence Microscopy Tracing

Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 supports fluorescence microscopy applications where far-red emission helps reduce background from common biological autofluorescence. Lab teams use Cy5-labeled biomolecules to visualize distribution, uptake, and localization patterns in fixed samples and labeled biological assemblies, including labeled macromolecular probes and surface-associated ligands. The dye's sulfonated character helps maintain aqueous solubility during staining and wash steps, which is particularly useful for multicolor imaging workflows that require reliable far-red channel performance.

3. Flow Cytometry Dye Conjugates

Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 is applied in flow cytometry development for generating far-red fluorescent conjugates used to quantify labeled biomolecules and binding events on cells. Researchers often prepare Cy5-tagged reagents for staining workflows where stable aqueous handling and strong far-red emission are required to discriminate signal from background. This dye is also used in assay development settings to support panel design for multiparameter cytometry, leveraging its emission into common far-red detection windows for robust gating and comparative quantification across experimental conditions.

4. Biomaterial Surface Functionalization

Sulfo-Bis-(N,N'-carboxylic acid)-Cy5 is frequently incorporated into biomaterial and polymer surface functionalization strategies to create fluorescently traceable materials for materials science and biomaterials research. Teams use Cy5-labeled coatings or tethered dye conjugates to monitor surface coverage, track material interactions with biological components, and visualize adsorption or immobilization behavior under fluorescence microscopy. The dye's carboxylate functionality supports attachment approaches that yield durable fluorescent labeling on engineered surfaces, enabling researchers to connect material processing variables with observable fluorescence distribution patterns.