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Sulfo-Cyanine5 maleimide | CAS 2242791-82-6
| Catalog Number | F03-0008 |
| Category | sulfo-Cyanine5 |
| Molecular Formula | C38H43KN4O9S2 |
| Molecular Weight | 803.00 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.An analog without sulfo groups, Cyanine5 maleimide, is also available.
Chemical Information
Product Specification
Application
| Related CAS | 2130955-10-9 (inner salt) 2242791-81-5 (inner salt) 2301999-22-2 (sodium salt) |
| Purity | NMR 1H, HPLC-MS (95+%) |
| Solubility | soluble in water, DMSO, DMF |
| Appearance | dark blue powder |
| ε, L⋅mol-1⋅cm-1 | 271000 |
| Fluorescence Quantum Yield | 0.28 |
| Excitation | 646 |
| Emission | 662 |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sulfo-Cyanine5 maleimide, a versatile fluorescent dye, finds widespread use in biological research owing to its vibrant fluorescence and reactive maleimide group. Here are four key applications of Sulfo-Cyanine5 maleimide:
Fluorescence Labeling: Widely employed for labeling proteins, peptides, and other biomolecules, Sulfo-Cyanine5 maleimide interacts specifically with thiol groups on cysteine residues, ensuring stable and precise conjugation. This capability is particularly advantageous in live-cell imaging, flow cytometry, and fluorescence microscopy, providing a powerful tool to monitor and visualize molecular interactions in diverse biological contexts.
Protein-Protein Interaction Studies: Leveraging Sulfo-Cyanine5 maleimide, researchers delve into the intricate world of protein-protein interactions through advanced techniques like Förster Resonance Energy Transfer (FRET). By labeling interacting proteins with distinct fluorophores, changes in fluorescence intensity can be scrutinized, offering insights into binding events and interaction dynamics. This approach sheds light on the intricate molecular mechanisms governing complex biological processes.
Quantitative Western Blotting: In the realm of quantitative Western blotting, Sulfo-Cyanine5 maleimide plays a pivotal role in providing accurate and sensitive protein detection. Following protein labeling on the blot, fluorescent signals are quantified using sophisticated imaging systems, enabling precise measurement of protein levels. This precision facilitates in-depth investigations into protein expression, degradation pathways, and post-translational modifications, advancing our understanding of cellular processes.
In Vivo Imaging: Sulfo-Cyanine5 maleimide emerges as a crucial tool in in vivo imaging studies, enabling real-time tracking of biomolecules and cells within living organisms. Its near-infrared fluorescence properties minimize background autofluorescence, facilitating deep tissue penetration for enhanced imaging depth. Particularly valuable for monitoring cellular trafficking, tumor imaging, and studying dynamic biological processes within animal models, this application showcases the versatility and utility of Sulfo-Cyanine5 maleimide in cutting-edge research endeavors.
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