TAMRA-C6-Acid | CAS 2183473-11-0

TAMRA-C6-Acid is a TAMRA-based fluorescent labeling reagent featuring a carboxylic acid handle for conjugation to amines and other nucleophilic functional groups, enabling red-orange fluorescence tracking in biochemical and imaging workflows. Its dye scaffold is commonly used as a robust visible-spectrum reporter in fluorescence microscopy, assay development, and nucleic-acid or protein labeling strategies where TAMRA emission is compatible with standard filter sets and plate readers.

1. Protein And Peptide Labeling

TAMRA-C6-Acid is frequently used by protein and peptide chemistry teams to generate fluorescent conjugates for binding studies, localization experiments, and fluorescence-based bioassays. Researchers typically incorporate the carboxylic-acid functionality into amide-forming labeling workflows to attach TAMRA to lysine-containing proteins, peptides, or peptide-tagged biomolecules, producing stable fluorescent probes for monitoring interactions, washing behavior, and binding kinetics in solution or on surfaces. In molecular imaging and assay development, TAMRA conjugates are also used as reference dyes to verify labeling efficiency and to standardize fluorescence readouts across experimental batches.

2. Fluorescent Oligonucleotide Tagging

TAMRA-C6-Acid is used in oligonucleotide labeling workflows where a red-emitting fluorophore is needed for fluorescence detection of nucleic-acid constructs. Molecular biology laboratories often employ the dye as a reporter for labeled primers, probes, or internal oligonucleotide tags in hybridization and nucleic-acid analysis formats, including fluorescence readouts on gel imaging systems and plate-based assays. The carboxylic acid functional group supports incorporation into oligonucleotide conjugates through established linker strategies, enabling researchers to track strand behavior, hybridization events, and sample processing steps with a TAMRA-compatible spectral signature.

3. Fluorescence Microscopy Tracing

TAMRA-C6-Acid supports cellular and biomaterials imaging workflows where red-orange fluorescence is advantageous for multicolor experiments and for tracking labeled components in microscopy. Researchers label biomolecules that associate with membranes, extracellular matrices, or engineered materials to visualize distribution patterns during incubation, washing, and time-course experiments. In fluorescence microscopy, TAMRA conjugates derived from TAMRA-C6-Acid are commonly used to assess uptake or binding to immobilized targets, to map reagent localization in labeled complexes, and to provide a consistent fluorescent tracer for comparing experimental conditions under the same imaging settings.