Sulfo DBCO-PEG4-amine | CAS 2055198-05-3

Sulfo DBCO-PEG4-amine is a water-soluble DBCO (dibenzocyclooctyne) click chemistry reagent bearing a PEG4 linker and a terminal amine, designed for rapid strain-promoted azide–alkyne cycloaddition (SPAAC) under bioconjugation conditions. The sulfonate functionality improves aqueous handling, while the PEG spacer helps reduce steric effects and supports uniform labeling of biomolecules, surfaces, and soft materials. This combination makes Sulfo DBCO-PEG4-amine well-suited for constructing azide-functional probes and conjugates used across chemical biology, molecular imaging workflows, and diagnostic reagent development.

1. Antibody And Protein Labeling

Sulfo DBCO-PEG4-amine is used to introduce a DBCO handle into protein-based reagents and labeling schemes where subsequent coupling to azide-bearing partners is required. The PEG4 spacer and terminal amine support practical conjugation design, enabling attachment strategies that preserve protein solubility and reduce steric hindrance during downstream SPAAC coupling. Researchers commonly employ this reagent to prepare azide-reactive labeling components for immunoassays, affinity probes, and protein tracking tools, particularly when aqueous compatibility and reproducible conjugation performance are critical.

2. Surface And Bead Conjugation

Sulfo DBCO-PEG4-amine is frequently incorporated into workflows that functionalize solid supports such as polymer beads, chromatography media, and sensor surfaces for azide-directed capture or labeling. Its sulfonated, water-compatible character helps maintain effective surface chemistry in buffered environments, while the PEG4 chain supports accessibility of the DBCO group for efficient SPAAC with azide-functional ligands. This approach is widely used in assay development and analytical chemistry to generate reusable reagent platforms, including affinity capture materials and multiplexing-ready surface chemistries.

3. PEGylated Probe Construction

Sulfo DBCO-PEG4-amine supports the assembly of PEGylated chemical biology probes where controlled spacing between functional groups improves handling and reduces non-specific interactions. The PEG4 linker is particularly valuable when building multicomponent constructs that require reliable SPAAC coupling to azide-tagged reporters, targeting motifs, or imaging handles. In molecular imaging and research diagnostics development, this reagent is used to create well-defined probe architectures that maintain aqueous solubility and promote consistent conjugation geometry across batches.

4. Nucleic Acid And Biomolecule Conjugates

Sulfo DBCO-PEG4-amine is applied in bioconjugation strategies that require attaching DBCO-bearing functionality to biomolecules used in labeling and tracking experiments, including nucleic acid constructs and other macromolecular reagents bearing azide partners. The water solubility and flexible PEG4 spacing help accommodate the crowded environments typical of nucleic acid and biomolecular assemblies, supporting efficient SPAAC coupling after functionalization. Teams developing research-grade labeling reagents often select this reagent when they need robust aqueous compatibility and a functional handle that integrates readily into modular conjugation pipelines.