Sulfo-Cyanine7 amine | CAS 2236573-39-8

Sulfo-Cyanine7 amine is a water-soluble cyanine dye bearing a primary amine handle for covalent conjugation to biomolecules, polymers, and biomaterial surfaces. Its near-infrared fluorescence supports low-background optical readouts in fluorescence imaging workflows, where stable labeling and efficient dye incorporation are needed for microscopy, conjugate tracking, and fluorescence-based assay development.

1. Protein And Antibody Labeling

Sulfo-Cyanine7 amine is routinely used to generate near-infrared fluorescent protein conjugates for research-grade imaging and binding studies. Researchers employ the dye's primary amine functionality to incorporate Sulfo-Cyanine7 into antibodies, affinity reagents, and other protein constructs that will be visualized by fluorescence microscopy or quantified in fluorescence-based assays. The sulfonated, water-soluble character helps maintain labeling performance in aqueous buffers commonly used for immunoassays and cell-binding experiments, while the cyanine7 emission is leveraged for optical detection with reduced interference from visible autofluorescence.

2. Fluorescence Microscopy Tracing

Sulfo-Cyanine7 amine is applied as a fluorescent labeling reagent for cellular and biomolecular tracing experiments where near-infrared imaging is advantageous. In microscopy workflows, the dye is incorporated into proteins, peptides, or other targeting ligands to visualize binding and localization patterns on fixed samples or in imaging setups compatible with cyanine7 excitation/emission. Because the dye is designed for aqueous conjugation chemistry, it is frequently selected for preparing imaging reagents used in multi-color fluorescence experiments where spectral separation from common fluorophores is required.

3. Biomaterial Surface Functionalization

Sulfo-Cyanine7 amine is used to functionalize hydrophilic biomaterial surfaces and polymer materials with a fluorescent tag for surface mapping and material characterization. Materials scientists incorporate the dye into coatings, hydrogels, or surface-modified scaffolds to enable visualization of immobilized biomolecules and to monitor coating uniformity or retention after washing and processing. The dye's amine functionality supports straightforward covalent attachment strategies to amine-reactive surface chemistries used in biomaterials development, while the near-infrared fluorescence supports detection in environments where visible dyes may suffer from higher background.

4. Fluorescence Bioassay Reagents

Sulfo-Cyanine7 amine serves as a building block for fluorescence-based assay reagents that rely on near-infrared signal readout. Assay developers use dye-labeled proteins or dye-containing conjugates to create fluorescent reporters for binding assays, reagent tracking, and plate-based quantification workflows. The water solubility and cyanine7 spectral region make it a practical choice when assay formats require aqueous compatibility and optical detection using standard fluorescence plate readers or imaging instruments configured for cyanine7 channels.