ROX azide, 6-isomer | CAS 1422178-12-8

ROX azide, 6-isomer is a ROX-based azide click reagent designed for fluorescent labeling workflows where a reactive azide handle is required. As a fluorescent azide, it enables post-labeling conjugation through azide-compatible click strategies while providing a red/orange emission signal for microscopy and fluorescence assay development. The ROX chromophore is commonly used in multicolor fluorescence experiments, where robust spectral separation from green channels is advantageous.

1. Fluorescent Click Labeling

ROX azide, 6-isomer is used as a fluorescent azide building block for labeling biomolecules, including proteins, peptides, and nucleic-acid-containing constructs, in click chemistry workflows. Researchers incorporate the azide functionality into target scaffolds and then perform azide-alkyne conjugation to attach the ROX fluorophore to the desired biomolecule or probe format. This approach is frequently adopted in chemical biology to generate defined fluorescent conjugates for downstream imaging, binding studies, and fluorescence readouts.

2. ROX Imaging Probe Construction

ROX azide, 6-isomer supports the construction of ROX-tagged imaging reagents used in fluorescence microscopy and related cellular imaging experiments. By providing a reactive azide group alongside a ROX fluorophore, the reagent can be integrated into probe designs where the fluorescent label needs to be installed at a specific stage of the workflow, such as after biomolecule assembly or surface coupling. Typical use includes generating fluorescently labeled ligands, tracking reagents, and multicomponent imaging tools where ROX emission provides convenient channel placement.

3. Multicolor Assay Development

ROX azide, 6-isomer is applied in fluorescence-based assay development where ROX labeling is leveraged for red/orange-channel detection in plate formats or instrument-based fluorescence measurements. Teams developing fluorescence assays often use ROX-conjugated biomolecular reagents to monitor binding events, reagent localization, or labeling efficiency in experimental pipelines that involve sequential or modular conjugation steps. The azide functionality helps streamline reagent assembly by enabling click-based attachment of the ROX label to assay components without requiring direct dye incorporation into every target during synthesis.

4. Biomaterial And Surface Functionalization

ROX azide, 6-isomer is used to introduce a fluorescent ROX tag into biomaterial and surface functionalization workflows that rely on click-compatible coupling. Material scientists and biointerface researchers incorporate the azide-bearing fluorescent reagent into polymers, coatings, or hydrogel systems and then use click conjugation to attach the ROX label to specific surface motifs or capture reagents. The resulting ROX-functional materials are commonly used for fluorescence visualization of surface modification, reagent immobilization, and spatial mapping in materials and interface studies.