ROX alkyne, 6-isomer | CAS 2097422-22-3

ROX alkyne, 6-isomer is a ROX-based alkyne click handle designed for fluorescent labeling workflows that require copper-catalyzed azide-alkyne cycloaddition (CuAAC). Its ROX fluorophore enables red-emitting detection in fluorescence imaging and plate-based assays, while the alkyne functionality supports rapid attachment to azide-bearing biomolecules, linkers, and materials. Researchers commonly use this reagent to build fluorescent conjugates for microscopy, flow cytometry, and assay development where a defined ROX dye payload is needed.

1. Biomolecule Click Labeling

ROX alkyne, 6-isomer is used to fluorescently tag proteins, peptides, and other azide-functional biomolecules through CuAAC, producing stable ROX-labeled conjugates for downstream imaging and analytical workflows. Chemical biology groups and assay developers rely on the alkyne handle to introduce a red-emitting ROX reporter onto targeting ligands, affinity reagents, or detection constructs that already carry an azide group. This approach is particularly common when the labeling needs to be modular, allowing the ROX dye to be swapped onto different azide-bearing partners without redesigning the entire conjugate.

2. Fluorescent Probe Construction

ROX alkyne, 6-isomer supports fluorescent probe development where a ROX fluorophore is incorporated as a reporter for readout in microscopy and fluorescence-based bioassays. Probe builders use the alkyne for click assembly of ROX-labeled scaffolds, including multicomponent sensing reagents, fluorescent tracking tags, and modular imaging reagents that can be coupled to azide-functional recognition elements or linkers. The result is a ROX-containing probe format that can be integrated into experimental platforms that monitor red fluorescence for localization, kinetics, or endpoint measurements.

3. Biomaterial And Surface Functionalization

ROX alkyne, 6-isomer is applied in biomaterials research to introduce a fluorescent ROX signal onto azide-functional surfaces, hydrogels, and polymer matrices via click chemistry. Materials scientists and surface engineers use the alkyne handle to generate ROX-labeled coatings or bulk-labeled materials that enable visualization of material distribution, surface coverage, and conjugate immobilization in fluorescence microscopy. This labeling strategy is also used to create fluorescently traceable material components for studying interactions at interfaces, such as attachment and retention of functionalized biomolecules on engineered substrates.

4. Flow Cytometry Dye Conjugates

ROX alkyne, 6-isomer is used to prepare ROX-containing conjugates for flow cytometry workflows that monitor red fluorescence from dye-labeled biomolecules. In cell labeling and reagent development, researchers click-attach the ROX alkyne to azide-bearing antibodies, binders, or labeling reagents to generate consistent fluorescent reagents for staining panels and fluorescence-based readouts. The ROX reporter enables multiplex-friendly experimental designs when instrument channels are configured for red emission detection, supporting quantitative analysis of labeled targets in suspension assays.