diSulfo-Cy3 azide | CAS 2055138-89-9

diSulfo-Cy3 azide is a sulfonated cyanine azide designed for copper-free click chemistry workflows that generate intensely colored, water-soluble Cy3-based conjugates. Its azide handle enables efficient attachment of the Cy3 fluorophore to biomolecules, polymers, and surfaces bearing complementary click partners, supporting fluorescence imaging and labeling workflows where aqueous compatibility is important. Researchers commonly use this reagent to build fluorescent conjugates for microscopy, cell analysis, and assay development with a red-emitting dye reporting on the location or presence of the tagged component.

1. Biomolecule Labeling

diSulfo-Cy3 azide is frequently used by chemical biology and glycobiology groups to fluorescently label proteins, peptides, and other biomolecules through click-based conjugation. The sulfonated Cy3 framework helps maintain aqueous handling for labeling reactions and downstream imaging buffers, enabling preparation of fluorescent conjugates for tracking binding partners, monitoring reagent distribution, and visualizing labeled biomolecules in microscopy experiments. This format is also commonly chosen when a modular fluorophore attachment strategy is needed to attach Cy3 to a biomolecule without requiring dye-specific labeling kits.

2. Fluorescence Microscopy

diSulfo-Cy3 azide supports fluorescence microscopy workflows where red-channel visualization of click-labeled targets is required. Investigators use Cy3 conjugates prepared from this azide to stain or tag cellular components, extracellular biomaterials, and affinity reagents in imaging studies, leveraging the bright Cy3 emission for clear visualization against typical background fluorescence. The reagent's azide functionality also makes it practical for constructing imaging probes from biomolecule scaffolds that have been functionalized with a complementary click handle, facilitating consistent probe-to-probe labeling in microscopy pipelines.

3. Flow Cytometry Dye Conjugates

diSulfo-Cy3 azide is used in flow cytometry development to generate Cy3-tagged reagents for fluorescent readouts of labeled binding events. Researchers prepare click-labeled antibodies, ligands, or other affinity constructs carrying the Cy3 reporter to quantify cell-associated fluorescence in single-cell analysis workflows. The water-soluble, sulfonated dye format is particularly convenient for preparing conjugates that must remain stable in aqueous staining buffers used in cytometry assays, supporting multiplex panel design where Cy3 provides a distinct red emission channel.

4. Surface And Polymer Functionalization

diSulfo-Cy3 azide is also applied in biomaterials and materials research to create Cy3-functionalized surfaces and polymer materials via click-based immobilization. Teams working on hydrogels, coatings, and engineered interfaces use Cy3 incorporation to visualize material distribution, confirm surface modification, or generate fluorescent reporters embedded in polymer matrices. The azide handle enables modular attachment to click-reactive substrates, allowing researchers to tune labeling density and spatial patterning for fluorescence-based characterization of functional materials.