AF594 activated ester, 5-isomer

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AF594 activated ester, 5-isomer

AF594 activated ester, 5-isomer | CAS 1638544-48-5

Catalog Number R01-0042
Category Alexa Fluor
Molecular Formula C51H67N5O13S2
Molecular Weight 1022.23
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Product Introduction

AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is the best alternative to Texas Red™, so it is commonly used for flow cytometry and fluorescent microscopy.,AF594 activated ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.,AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.

  • Chemical Information

  • Product Specification

  • Application

Purity NMR 1H, HPLC-MS (95%)
Solubility Soluble in water, DMSO, DMF
Storage Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

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