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AF594 activated ester, 5-isomer | CAS 1638544-48-5
| Catalog Number | R01-0042 |
| Category | Alexa Fluor |
| Molecular Formula | C51H67N5O13S2 |
| Molecular Weight | 1022.23 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is the best alternative to Texas Red™, so it is commonly used for flow cytometry and fluorescent microscopy.,AF594 activated ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.,AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.
Chemical Information
Product Specification
Application
| Purity | NMR 1H, HPLC-MS (95%) |
| Solubility | Soluble in water, DMSO, DMF |
| Storage | Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
AF594 activated ester 5-isomer, a versatile fluorescent dye, finds wide-ranging utility in biochemistry and molecular biology. Here are four key applications of AF594 activated ester 5-isomer:
Fluorescent Labeling: In the realm of biomolecular research, AF594 activated ester 5-isomer serves as a potent tool for fluorescently tagging various biomolecules, including proteins, antibodies, and nucleic acids. This labeling strategy allows researchers to visually track and observe these entities within live cells or tissue specimens using the powerful tool of fluorescence microscopy. The intense brightness and robust stability of this dye make it a preferred option for conducting prolonged imaging studies, shedding light on dynamic processes within biological systems.
Flow Cytometry: Venturing into the domain of flow cytometry, AF594 activated ester 5-isomer emerges as a pivotal component for conjugation with antibodies or other probes to interrogate cell populations. This conjugation facilitates the identification and quantification of specific markers present on cell surfaces or within cells, enabling a deeper understanding of cellular diversity and functionalities. The distinctive fluorescent signal emitted by AF594 contributes to multicolor flow cytometric analysis, enabling the exploration of intricate cellular phenotypes and paving the way for comprehensive cellular investigations.
Fluorescence Resonance Energy Transfer (FRET): Delving into the realm of molecular dynamics, AF594 activated ester 5-isomer plays a critical role in FRET experiments aimed at investigating molecular interactions and structural changes. When employed as either a donor or acceptor dye, it pairs with a compatible FRET partner to relay insights into the spatial proximity and dynamic alterations of interacting molecules. This technique serves as a valuable tool for dissecting protein-protein interactions and elucidating other complex biomolecular processes, offering a window into the intricate choreography of cellular mechanisms.
Immunofluorescence: Widely embraced in immunological studies, AF594 activated ester 5-isomer stands as a key player in immunofluorescence assays dedicated to uncovering specific antigens within cellular contexts or tissue sections. By coupling the dye with primary or secondary antibodies, researchers gain the ability to visualize and map the distribution patterns and expression levels of target proteins, unraveling the intricate tapestry of cellular structures, signaling cascades, and disease-associated alterations.
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