AF430 NHS ester | CAS 467233-94-9

AF430 NHS ester is an N-hydroxysuccinimide (NHS) ester fluorophore designed for efficient covalent labeling of primary amines on proteins, peptides, and other amine-containing biomolecules. Its reactive NHS ester enables straightforward preparation of fluorescent conjugates for fluorescence imaging and quantitative fluorescence readouts, while the AF430 emission supports detection in standard visible-light fluorescence workflows.

1. Protein Fluorescent Labeling

AF430 NHS ester is widely used to generate fluorescently labeled proteins for microscopy, gel-based fluorescence imaging, and fluorescence-based binding studies. Researchers typically target lysine residues and N-terminal amines on antibodies, enzymes, and scaffold proteins to create stable amine-reactive conjugates for tracking biomolecule localization, monitoring binding interactions, or visualizing protein distribution in assay formats that rely on fluorescence intensity.

2. Antibody Conjugate Preparation

AF430 NHS ester supports preparation of antibody conjugates used in immunofluorescence and fluorescence immunoassays where covalent attachment to antibody lysines is required. Lab groups commonly employ it to produce fluorescent secondary reagents, labeled primary antibodies, or antibody-based probes for cell-surface or extracellular targets in workflows that read out AF430 fluorescence using standard fluorescence microscopes or plate readers.

3. Peptide And Biomolecule Tagging

AF430 NHS ester is also applied to label peptides and other amine-bearing biomolecules, enabling fluorescent tagging for affinity reagents, tracer molecules, and biomolecule tracking experiments. In chemical biology and biomaterials research, it is frequently used to create fluorescent conjugates that can be incorporated into downstream studies such as adsorption/interaction assessments, component tracking in complex mixtures, and visualization of labeled building blocks within experimental systems.

4. Surface And Material Functionalization

AF430 NHS ester can be used to functionalize amine-containing surfaces and biomaterials, including polymer coatings and amine-derivatized substrates, to create fluorescently marked interfaces. Materials scientists and surface chemistry users often incorporate AF430-labeled components to visualize coating uniformity, track immobilized biomolecules, and support fluorescence readouts in assays where spatial mapping of functionalized surfaces is required.