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AF568 NHS ester
| Catalog Number | R01-0041 |
| Category | AF |
| Molecular Formula | C49H63N5O13S2 |
| Molecular Weight | 994.18 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R01-0041 | -- | $-- |
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Product Introduction
AF568 is a synthetic fluorophore. The excitation peak of AF568 lies at 572 nm and its emission peak is at 598 nm. It can be excited using a 561 nm laser. AF568 is spectrally similar to TF4 (Tide Fluor™ 4), and sulfo-Cyanine3.5 dyes.,As AF568 NHS Ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.
Chemical Information |
|
|---|---|
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | 5-(2,5-dioxopyrrolidin-1-yl)oxycarbonyl-2-[7,7,19,19-tetramethyl-9,17-bis(sulfonatomethyl)-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl]benzoate;triethylazanium |
| Canonical SMILES | CC[NH+](CC)CC.CC[NH+](CC)CC.CC1(C=C(C2=CC3=C(C=C2N1)OC4=CC5=[NH+]C(C=C(C5=CC4=C3C6=C(C=C(C=C6)C(=O)ON7C(=O)CCC7=O)C(=O)[O-])CS(=O)(=O)[O-])(C)C)CS(=O)(=O)[O-])C |
| InChI | InChI=1S/C37H33N3O13S2.2C6H15N/c1-36(2)14-19(16-54(46,47)48)22-10-25-29(12-27(22)38-36)52-30-13-28-23(20(17-55(49,50)51)15-37(3,4)39-28)11-26(30)33(25)21-6-5-18(9-24(21)34(43)44)35(45)53-40-31(41)7-8-32(40)42;2*1-4-7(5-2)6-3/h5-6,9-15,38H,7-8,16-17H2,1-4H3,(H,43,44)(H,46,47,48)(H,49,50,51);2*4-6H2,1-3H3 |
| InChI Key | YRFFGLVXBMUQQI-UHFFFAOYSA-N |
| Solubility | Good in water, DMSO, DMF |
| Appearance | Dark colored solid |
- Product Specification
| CF260 | 0.4 |
| CF280 | 0.32 |
| Fluorescence Quantum Yield | 0.912 |
| Mass Spec M+ Increment | 676.1 |
| Excitation | 572 |
| Emission | 598 |
| Storage | Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Computed Properties | |
|---|---|
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 14 |
| Rotatable Bond Count | 12 |
| Exact Mass | 993.38637943 g/mol |
| Monoisotopic Mass | 993.38637943 g/mol |
| Topological Polar Surface Area | 279Ų |
| Heavy Atom Count | 69 |
| Formal Charge | 0 |
| Complexity | 2040 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 3 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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