N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide | CAS 872979-87-8

N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide is a cationic polymethine dye used as a fluorescence labeling and imaging reagent where strong visible emission and electrostatic association with negatively charged biomaterials can be leveraged. Its permanently charged trimethylammonium group supports aqueous handling and interaction with biological polymers, while the extended conjugation enables bright fluorescence for optical readouts in microscopy and fluorescence-based assays.

1. Fluorescence Microscopy Staining

N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide is used in fluorescence microscopy workflows to visualize and track labeled biomolecular assemblies, including nucleic-acid-containing structures and other negatively charged components in fixed or prepared samples. Researchers commonly employ it as a staining dye to generate high-contrast fluorescence images when optical excitation and emission match standard visible-light microscope filter sets, enabling localization studies in cell biology, materials biology, and bioimaging development.

2. Nucleic Acid Labeling Assays

N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide is applied as a fluorescent probe for nucleic acid labeling in bench-top assay development, where cationic dyes are used to report on nucleic-acid presence and distribution. In laboratory settings, it is frequently incorporated into fluorescence workflows for qualitative visualization of DNA/RNA in gels, membranes, or microfluidic formats, supporting reagent optimization during nucleic acid handling and analytical method development.

3. Fluorescence-Based Bioassay Readouts

N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide supports fluorescence readouts in assay formats that rely on dye binding to charged biological matrices. Developers use it in plate-based or cuvette-based experiments where the intensity of the dye-associated signal provides a convenient optical endpoint for monitoring sample composition, nucleic-acid-containing reagents, or other negatively charged macromolecular environments, with detection carried out on common fluorescence plate readers.

4. Biomaterial and Polymer Imaging

N-(3-Trimethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide is also used to visualize charged biomaterials and polymeric constructs in imaging studies, including electrostatically assembled films and scaffolds. Materials scientists and chemical biologists incorporate the dye to map distribution within polymer networks or to confirm labeling uniformity in composite systems, taking advantage of the dye's cationic character and fluorescent output for optical characterization of engineered biomaterials.