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Mag-fura-2 tetrapotassium salt | CAS 132319-57-4
| Catalog Number | A14-0073 |
| Category | Calcium, Chloride and Other indicators |
| Molecular Formula | C18H10K4N2O11 |
| Molecular Weight | 586.68 |
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Product Introduction
Mag-fura-2 tetrapotassium salt is a ratiometric fluorescent probe utilized in the detection and measurement of intracellular magnesium ions. As part of the fura family of dyes, it features a benzofuran chromophore that exhibits shifts in excitation wavelengths upon binding to magnesium, allowing for precise quantification through fluorescence microscopy or spectrofluorometry. The tetrapotassium salt form enhances its solubility in aqueous environments, facilitating its incorporation into live cell imaging assays and enabling the study of magnesium-dependent physiological processes.
Chemical Information
Application
Computed Properties
Patents
Chemical Information
| Related CAS | 120551-15-7 (free base) |
| Synonyms | Furaptra Tetrapotassium Salt; Fura 2 magnesium-selective analog tetrapotassium salt; 2-[6-[bis(carboxylatomethyl)amino]-5-(carboxylatomethoxy)-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate tetrapotassium salt |
| IUPAC Name | tetrapotassium;2-[6-[bis(carboxylatomethyl)amino]-5-(carboxylatomethoxy)-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate |
| SMILES | C1=C2C=C(OC2=CC(=C1OCC(=O)[O-])N(CC(=O)[O-])CC(=O)[O-])C3=NC=C(O3)C(=O)[O-].[K+].[K+].[K+].[K+] |
| InChI | InChI=1S/C18H14N2O11.4K/c21-14(22)5-20(6-15(23)24)9-3-10-8(1-11(9)29-7-16(25)26)2-12(30-10)17-19-4-13(31-17)18(27)28;;;;/h1-4H,5-7H2,(H,21,22)(H,23,24)(H,25,26)(H,27,28);;;;/q;4*+1/p-4 |
| InChIKey | PQSIPSUBCVAWSY-UHFFFAOYSA-J |
| Appearance | Solid Powder |
Application
Mag-fura-2 tetrapotassium salt, a fluorescent calcium indicator widely applied in biochemistry and cell biology, possesses diverse uses. Here are four key applications:
Intracellular Calcium Measurement: Employing Mag-fura-2 tetrapotassium salt facilitates the precise measurement of intracellular calcium concentrations in live cells. Its fluorescence properties undergo alterations upon calcium binding, enabling real-time monitoring of calcium dynamics via fluorescence microscopy or flow cytometry. This method is imperative for investigating calcium signaling pathways and their multifaceted roles in various cellular processes.
Neuroscience Research: Within the realm of neuroscience, Mag-fura-2 tetrapotassium salt serves as a valuable tool for delving into calcium flux in neurons and glial cells. By tracking calcium levels, researchers can delve into neuronal activity, synaptic transmission, and calcium-dependent signaling in the brain. This aids in unraveling the intricate mechanisms governing neural communication, plasticity, and neurodegenerative ailments.
Cardiac Physiology: In the domain of cardiac physiology, Mag-fura-2 tetrapotassium salt proves invaluable for tracking calcium transients in cardiac muscle cells. Precise measurement of intracellular calcium is crucial for comprehending excitation-contraction coupling and cardiac function. Researchers utilize this indicator to scrutinize the impacts of drugs and genetic modifications on cardiac calcium handling, offering insights pertinent to heart disease research.
Pharmacological Screening: This fluorescent calcium indicator finds utility in high-throughput screening for drug discovery purposes. By assessing calcium responses in cell-based assays, it aids in the identification of compounds influencing calcium-dependent pathways. Such an application holds particular significance in screening potential drugs targeting calcium channels, receptors, and other associated proteins.
Computed Properties
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 13 |
| Rotatable Bond Count | 6 |
| Exact Mass | 585.8832851 g/mol |
| Monoisotopic Mass | 585.8832851 g/mol |
| Topological Polar Surface Area | 212Ų |
| Heavy Atom Count | 35 |
| Formal Charge | 0 |
| Complexity | 679 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 5 |
| Compound Is Canonicalized | Yes |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| US-2003180964-A1 | Method for immobilizing analyte sensitive materials on a sol-gel matrix | 2002-03-20 |
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