Mag-fura-2 tetrapotassium salt | 132319-57-4

Catalog Number	A14-0073
Category	Calcium, Chloride Indictors and Other indicators
Molecular Formula	C18H10K4N2O11
Molecular Weight	586.68

Catalog Number	Size	Price	Quantity
A14-0073	--	$--

inquiry

Worldwide Delivery
Quality Assurance
24/7 Customer Service

Product Introduction

Mag-fura-2 tetrapotassium salt is a cell-impermeant fluorescent magnesium indicator. Mag-fura-2 tetrapotassium salt may be loaded into live cells by microinjection, patch pipette infusion, scrape loading, bead loading, or ATP-induced permeabilization. Intracellular magnesium concentration can be measured by ratio of fluorescence intensity generated by mag-fura-2.

Chemical Information
Related CAS	120551-15-7 (free base)
Synonyms	Furaptra Tetrapotassium Salt; Fura 2 magnesium-selective analog tetrapotassium salt; 2-[6-[bis(carboxylatomethyl)amino]-5-(carboxylatomethoxy)-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate tetrapotassium salt
IUPAC Name	tetrapotassium;2-[6-[bis(carboxylatomethyl)amino]-5-(carboxylatomethoxy)-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate
Canonical SMILES	C1=C2C=C(OC2=CC(=C1OCC(=O)[O-])N(CC(=O)[O-])CC(=O)[O-])C3=NC=C(O3)C(=O)[O-].[K+].[K+].[K+].[K+]
InChI	InChI=1S/C18H14N2O11.4K/c21-14(22)5-20(6-15(23)24)9-3-10-8(1-11(9)29-7-16(25)26)2-12(30-10)17-19-4-13(31-17)18(27)28;;;;/h1-4H,5-7H2,(H,21,22)(H,23,24)(H,25,26)(H,27,28);;;;/q;4*+1/p-4
InChI Key	PQSIPSUBCVAWSY-UHFFFAOYSA-J
Appearance	Solid Powder

Application

Mag-fura-2 tetrapotassium salt, a fluorescent calcium indicator widely applied in biochemistry and cell biology, possesses diverse uses. Here are four key applications:

Intracellular Calcium Measurement: Employing Mag-fura-2 tetrapotassium salt facilitates the precise measurement of intracellular calcium concentrations in live cells. Its fluorescence properties undergo alterations upon calcium binding, enabling real-time monitoring of calcium dynamics via fluorescence microscopy or flow cytometry. This method is imperative for investigating calcium signaling pathways and their multifaceted roles in various cellular processes.

Neuroscience Research: Within the realm of neuroscience, Mag-fura-2 tetrapotassium salt serves as a valuable tool for delving into calcium flux in neurons and glial cells. By tracking calcium levels, researchers can delve into neuronal activity, synaptic transmission, and calcium-dependent signaling in the brain. This aids in unraveling the intricate mechanisms governing neural communication, plasticity, and neurodegenerative ailments.

Cardiac Physiology: In the domain of cardiac physiology, Mag-fura-2 tetrapotassium salt proves invaluable for tracking calcium transients in cardiac muscle cells. Precise measurement of intracellular calcium is crucial for comprehending excitation-contraction coupling and cardiac function. Researchers utilize this indicator to scrutinize the impacts of drugs and genetic modifications on cardiac calcium handling, offering insights pertinent to heart disease research.

Pharmacological Screening: This fluorescent calcium indicator finds utility in high-throughput screening for drug discovery purposes. By assessing calcium responses in cell-based assays, it aids in the identification of compounds influencing calcium-dependent pathways. Such an application holds particular significance in screening potential drugs targeting calcium channels, receptors, and other associated proteins.

Computed Properties
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	13
Rotatable Bond Count	6
Exact Mass	585.8832851 g/mol
Monoisotopic Mass	585.8832851 g/mol
Topological Polar Surface Area	212Å²
Heavy Atom Count	35
Formal Charge	0
Complexity	679
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	5
Compound Is Canonicalized	Yes