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Indo-1 AM | 112926-02-0
| Catalog Number | A14-0026 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C47H51N3O22 |
| Molecular Weight | 1009.9 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0026 | -- | $-- |
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Product Introduction
Indo-1 AM is a cell-permeant acetoxymethyl ester of indo-1, a ratiometric fluorescent calcium indicator. Indo-1 AM is hydrolyzed by intracellular esterases to release Indo-1 in cells. Indo-1 is commonly used in flow cytometry. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.
Chemical Information |
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| Synonyms | Indo-1 Acetoxymethyl ester; Indo-1 pentaacetoxymethyl ester; 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-Indole-6-carboxylic acid, (acetyloxy)methyl ester |
| Purity | ≥90% |
| Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChI | InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3 |
| InChI Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 349-364 nm |
| Emission | 475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound) |
| Storage | Store at -20°C |
Indo-1 AM, a fluorescent calcium indicator, plays a pivotal role in various bioscience research areas. Here are four key applications of Indo-1 AM:
Calcium Imaging in Live Cells: Widely utilized in live-cell imaging, Indo-1 AM enables the real-time monitoring of intracellular calcium levels. Its fluorescent properties allow researchers to vividly visualize and quantify calcium fluctuations, providing crucial insights into calcium signaling pathways, neurotransmitter release, and muscle contraction dynamics.
Cardiomyocyte Function Studies: Delving into the intricacies of cardiac muscle cells, Indo-1 AM facilitates the assessment of calcium dynamics within cardiomyocytes. By introducing Indo-1 AM into these cells, researchers can explore the calcium transients associated with cardiac muscle contractions and relaxations, offering essential perspectives on heart function and the underlying mechanisms of cardiac pathologies like arrhythmias.
High-Throughput Screening: A valuable tool in high-throughput screening assays, Indo-1 AM aids in the identification of compounds that modulate calcium signaling pathways. By swiftly measuring changes in fluorescence intensity, researchers can efficiently screen large compound libraries for their impacts on calcium levels, supporting drug discovery efforts and the development of novel therapeutic agents targeting calcium-dependent pathways.
Applications of Fluorescent Probes & Dyes
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