Indo-1 AM | 112926-02-0
Catelog Number | A14-0026 |
Category | Calcium, Chloride Indictors and Other indicators |
Molecular Formula | C47H51N3O22 |
Molecular Weight | 1009.9 |
Catalog Number | Size | Price | Quantity |
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A14-0026 | -- | $-- |
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Product Introduction
Indo-1 AM is a cell-permeant acetoxymethyl ester of indo-1, a ratiometric fluorescent calcium indicator. Indo-1 AM is hydrolyzed by intracellular esterases to release Indo-1 in cells. Indo-1 is commonly used in flow cytometry. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.
Chemical Information |
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Synonyms | Indo-1 Acetoxymethyl ester; Indo-1 pentaacetoxymethyl ester; 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-Indole-6-carboxylic acid, (acetyloxy)methyl ester |
Purity | ≥90% |
Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
InChI | InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3 |
InChI Key | CAWBRCOBJNWRLK-UHFFFAOYSA-N |
Appearance | Solid Powder |
Product Specification |
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Excitation | 349-364 nm |
Emission | 475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound) |
Storage | Store at -20°C |
Applications of Fluorescent Probes & Dyes
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