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Fluo-4 AM | 273221-67-3
| Catalog Number | A14-0005 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C51H50F2N2O23 |
| Molecular Weight | 1096.95 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0005 | -- | $-- |
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Product Introduction
Fluo-4 AM is a cell-permeable fluorescent Ca2+ indicator.
Chemical Information |
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|---|---|
| Synonyms | N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester |
| Purity | ≥95% by HPLC |
| IUPAC Name | acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-N-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate |
| Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChI | InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3 |
| InChI Key | QOMNQGZXFYNBNG-UHFFFAOYSA-N |
- Product Specification
- Application
| Excitation | 494 nm |
| Emission | 506 nm |
| Storage | Store at -20°C |
Fluo-4 AM, a fluorescent calcium indicator, plays a pivotal role in biological research as a tool for visualizing intracellular calcium levels. Here are four key applications of Fluo-4 AM:
Live Cell Imaging: Widely employed in live cell imaging, Fluo-4 AM enables the real-time monitoring of fluctuations in intracellular calcium levels. By introducing Fluo-4 AM into cells, researchers can track calcium dynamics using fluorescence microscopy, providing insights into dynamic cellular processes like signal transduction, muscle contraction, and neurotransmitter release with vivid clarity.
Drug Screening: In the realm of pharmacology, Fluo-4 AM serves as a reliable agent for screening compounds that influence calcium signaling pathways. High-throughput screening assays leverage Fluo-4 AM to detect changes in fluorescence indicative of alterations in calcium concentrations following drug treatments. This methodology proves invaluable for identifying potential pharmaceutical agents that modulate calcium-dependent cellular functions.
Neuroscience Research: At the forefront of neuroscience, Fluo-4 AM emerges as an indispensable asset for investigating calcium signaling in neurons. By visualizing calcium dynamics, scientists delve into the intricacies of neuronal activity, synaptic transmission, and plasticity, shedding light on the complexities of neurological diseases. This nuanced understanding aids in the development of targeted interventions for conditions such as epilepsy and neurodegenerative disorders.
Cardiovascular Studies: Essential for cardiac research, Fluo-4 AM facilitates the assessment of calcium handling in cardiac cells, particularly in cardiomyocytes. By tracking calcium transients, researchers unravel the mechanisms underpinning heart contraction and arrhythmias, providing crucial insights for developing therapeutic interventions in heart diseases. Additionally, this application illuminates the effects of various drugs on cardiac function, enhancing our comprehension of cardiac physiology and pathology.
Computed Properties | |
|---|---|
| XLogP3 | 5.5 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 27 |
| Rotatable Bond Count | 36 |
| Exact Mass | 1096.27724217 g/mol |
| Monoisotopic Mass | 1096.27724217 g/mol |
| Topological Polar Surface Area | 297Ų |
| Heavy Atom Count | 78 |
| Formal Charge | 0 |
| Complexity | 2220 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| US-2020399282-A1 | Inhibitors of trpc6 | 2018-02-15 |
| US-2019169167-A1 | Inhibitors of trpc6 | 2017-10-27 |
| US-2019169168-A1 | Inhibitors of trpc6 | 2017-10-27 |
| EP-3700902-A1 | Pyridine carbonyl derivatives and therapeutic uses thereof as trpc6 inhibitors | 2017-10-27 |
| US-10800757-B2 | Inhibitors of TRPC6 | 2017-10-27 |
Applications of Fluorescent Probes & Dyes
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