Fluo-3FF AM

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Fluo-3FF AM

Fluo-3FF AM | 348079-13-0

Catelog Number A14-0016
Category Calcium, Chloride Indictors and Other indicators
Molecular Formula C50H46Cl2F2N2O23
Molecular Weight 1151.8
Catalog Number Size Price Quantity
A14-0016 -- $--

Product Introduction

Fluo-3FF AM is a cell-permeable acetoxy-methyl ester of Fluo-3FF, a fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds = 42 and 0.4 µM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

Chemical Information

Synonyms N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-2,3-difluorophenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine(acetyloxy)methyl ester
Purity ≥95%
Canonical SMILES CC(=O)OCOC1=C(C=C2C(=C1)OC3=CC(=O)C(=CC3=C2C4=CC(=C(C=C4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC5=C(C=CC(=C5F)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)Cl)Cl
InChI InChI=1S/C50H46Cl2F2N2O23/c1-26(57)69-21-74-42-16-41-33(14-35(42)52)48(32-13-34(51)39(62)15-40(32)79-41)31-6-8-37(55(17-44(63)75-22-70-27(2)58)18-45(64)76-23-71-28(3)59)43(12-31)67-10-11-68-50-38(9-7-36(53)49(50)54)56(19-46(65)77-24-72-29(4)60)20-47(66)78-25-73-30(5)61/h6-9,12-16H,10-11,17-25H2,1-5H3
InChI Key ABZBPPQDKRLSCZ-UHFFFAOYSA-N
Appearance Solid Powder

Product Specification

Excitation 506 nm
Emission 526 nm
Storage Store at -20°C
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