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Fluo-3FF AM | 348079-13-0
| Catalog Number | A14-0016 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C50H46Cl2F2N2O23 |
| Molecular Weight | 1151.8 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0016 | -- | $-- |
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Product Introduction
Fluo-3FF AM is a cell-permeable acetoxy-methyl ester of Fluo-3FF, a fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds = 42 and 0.4 µM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.
Chemical Information |
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| Synonyms | N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-2,3-difluorophenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine(acetyloxy)methyl ester |
| Purity | ≥95% |
| Canonical SMILES | CC(=O)OCOC1=C(C=C2C(=C1)OC3=CC(=O)C(=CC3=C2C4=CC(=C(C=C4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC5=C(C=CC(=C5F)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)Cl)Cl |
| InChI | InChI=1S/C50H46Cl2F2N2O23/c1-26(57)69-21-74-42-16-41-33(14-35(42)52)48(32-13-34(51)39(62)15-40(32)79-41)31-6-8-37(55(17-44(63)75-22-70-27(2)58)18-45(64)76-23-71-28(3)59)43(12-31)67-10-11-68-50-38(9-7-36(53)49(50)54)56(19-46(65)77-24-72-29(4)60)20-47(66)78-25-73-30(5)61/h6-9,12-16H,10-11,17-25H2,1-5H3 |
| InChI Key | ABZBPPQDKRLSCZ-UHFFFAOYSA-N |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 506 nm |
| Emission | 526 nm |
| Storage | Store at -20°C |
Fluo-3FF AM, a fluorescent calcium indicator, finds extensive use in various biochemical and physiological applications. Here are four key applications of Fluo-3FF AM:
Calcium Imaging: An indispensable tool in calcium imaging, Fluo-3FF AM is widely utilized to monitor intracellular calcium levels in live cells. Upon loading cells with this dye, it emits fluorescence upon binding to calcium ions, enabling researchers to visualize and quantify calcium fluxes in real-time. This capability is vital for investigating intricate cellular processes, such as muscle contraction, neurotransmitter release, and signal transduction.
Drug Screening: Within the realm of pharmaceutical research, Fluo-3FF AM is a valuable asset for screening potential drug candidates that can modulate calcium channels or receptors. By tracking changes in calcium levels, researchers can evaluate the effectiveness and potency of drugs targeting calcium signaling pathways. This application plays a crucial role in the discovery and advancement of novel therapeutic agents.
Neuroscience Research: Essential for delving into calcium dynamics in neurons and brain tissues, Fluo-3FF AM holds a pivotal role in neuroscience research. Given the critical role of calcium signaling in neuron excitability, synaptic plasticity, and neurotransmission, researchers rely on this dye to explore neuronal activity patterns, synaptic interactions, and the impacts of neuroactive compounds on calcium homeostasis.
Cardiac Studies: In the realm of cardiac research, Fluo-3FF AM serves as a key tool for studying calcium handling in heart cells. Given the essential role of calcium ions in cardiac muscle contraction and relaxation, monitoring calcium dynamics is crucial for unraveling the complexities of heart function. By leveraging this indicator, scientists can investigate mechanisms underlying cardiac diseases, assess the effects of drugs, and explore cellular responses to various physiological and pathological stimuli.
Applications of Fluorescent Probes & Dyes
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