Fluo-3 AM | 121714-22-5

Catalog Number	A14-0002
Category	Calcium, Chloride and Other indicators
Molecular Formula	C51H50Cl2N2O23
Molecular Weight	1129.9

Catalog Number	Size	Price	Quantity
A14-0002	--	$--

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Product Introduction

Fluo-3 is a visable wavelength calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels. Fluo-3 AM is cell-permeable and can be cleaved to release fluo-3 in cells.

Chemical Information
Product Specification
Application

Synonyms	Fluo-3 Acetoxymethyl ester; N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine, (acetyloxy)methyl ester
Purity	≥90%
Canonical SMILES	CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)OCOC(=O)C)Cl)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChI	InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
InChI Key	PTPUOMXKXCCSEN-UHFFFAOYSA-N

Excitation	488 nm
Emission	526 nm
Storage	Store at -20°C

Fluo-3 AM is a fluorescent calcium indicator commonly used in various biological and medical research applications. Here are some key applications of Fluo-3 AM:

Calcium Imaging in Neuroscience: Fluo-3 AM is widely used in neuroscience research to visualize and measure calcium dynamics in neurons. By loading cells with Fluo-3 AM, researchers can monitor calcium transients in real-time under a fluorescence microscope. This technique helps in understanding neural activity, synaptic transmission, and the overall function of neural networks.

Cardiomyocyte Studies: In cardiology research, Fluo-3 AM is employed to study calcium signaling in cardiomyocytes, the heart muscle cells. Calcium fluxes are critical for heart muscle contraction and the regulation of cardiac rhythms. Researchers use Fluo-3 AM to investigate the effects of drugs, diseases, or genetic modifications on calcium dynamics within heart cells, providing insights into cardiac physiology and pathophysiology.

Drug Screening: Fluo-3 AM is used in high-throughput screening assays to identify compounds that modulate calcium levels in cells. In pharmaceutical research, potential drugs are tested for their effects on calcium signaling pathways using Fluo-3 AM-loaded cells. This application is particularly valuable in discovering new therapeutics for disorders related to calcium dysregulation, such as certain cardiovascular and neurodegenerative diseases.

Cell Physiology Studies: Fluo-3 AM helps researchers study calcium-dependent processes in various cell types, including muscle cells, immune cells, and cancer cells. Calcium ions play central roles in numerous cellular functions, such as muscle contraction, cell migration, and apoptosis. By monitoring changes in intracellular calcium levels with Fluo-3 AM, scientists can unravel the mechanisms behind these vital cellular activities and their alterations in disease conditions.

Applications of Fluorescent Probes & Dyes