Cy5-NHS ester | 146368-14-1

Catalog Number	F02-0026
Category	sulfo-Cyanine5
Molecular Formula	C37H43N3O10S2
Molecular Weight	753.882

Catalog Number	Size	Price
F02-0026	100 mg	$729
F02-0026	1 g	$3884
F02-0026	--	--

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Product Introduction

Cy5-NHS ester is a red-fluorescent probe that is reactive with primary amines on biomolecules such as proteins and nucleic acids.

Chemical Information
Product Specification
Application

Synonyms	Sulfo-Cyanine5, SE (ethyl);Cyanine 5, SE; 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, Inner Salt
Purity	98%
Canonical SMILES	CCN1C2=C(C=C(C=C2)S(=O)(=O)[O-])C(C1=CC=CC=CC3=[N+](C4=C(C3(C)C)C=C(C=C4)S(=O)(=O)O)CCCCCC(=O)ON5C(=O)CCC5=O)(C)C
InChI	InChI=1S/C37H43N3O10S2/c1-6-38-29-18-16-25(51(44,45)46)23-27(29)36(2,3)31(38)13-9-7-10-14-32-37(4,5)28-24-26(52(47,48)49)17-19-30(28)39(32)22-12-8-11-15-35(43)50-40-33(41)20-21-34(40)42/h7,9-10,13-14,16-19,23-24H,6,8,11-12,15,20-22H2,1-5H3,(H-,44,45,46,47,48,49)
InChI Key	WXWLHDCCGVWTDZ-UHFFFAOYSA-N

Excitation	649 nm
Emission	666 nm
Storage	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cy5-NHS ester, a fluorescent dye commonly utilized in biomolecular labeling, finds diverse applications in scientific research. Here are four key applications of Cy5-NHS ester:

Fluorescent Labeling of Proteins: Embracing Cy5-NHS ester, researchers covalently attach fluorescent labels to proteins, enabling the visualization and tracking of protein localization and interactions within cells. By targeting the amine groups on proteins, this technique facilitates detailed studies in confocal microscopy and flow cytometry. The bright and stable fluorescence exhibited by Cy5 elevates it as a prime choice for conducting intricate analyses on protein dynamics.

Nucleic Acid Probes: Conjugating Cy5-NHS ester to nucleic acids like DNA and RNA leads to the creation of highly sensitive probes with diverse applications in molecular biology. These probes, utilized in fluorescence in situ hybridization (FISH) and quantitative PCR, enable the precise detection and quantification of specific nucleic acid sequences. The exceptional signal-to-noise ratio of Cy5 ensures accurate and dependable detection in these crucial assays.

Biosensor Development: Playing a pivotal role in fluorescent biosensor development, Cy5-NHS ester equips scientists with tools to detect biological molecules and environmental conditions with unparalleled specificity. By labeling antibodies, enzymes, or other receptor molecules with Cy5, these biosensors generate measurable fluorescent signals upon binding to target analytes. This cutting-edge technology finds applications in clinical diagnostics, environmental monitoring, and food safety testing, offering insights into diverse fields.

Flow Cytometry: In the realm of flow cytometry, Cy5-NHS ester shines in facilitating multi-color analysis of cell populations. Its application in labeling cell surface markers enables intricate phenotypic analysis and cell sorting based on their expressions. Cy5’s compatibility with other fluorescent markers allows for comprehensive multi-parametric studies within complex cell mixtures, providing researchers with a holistic view of cellular dynamics.

Applications of Fluorescent Probes & Dyes