Coelenterazine H | 50909-86-9

Coelenterazine H is a bioluminescent substrate that finds extensive use in biochemical and biotechnological applications. Here are some key applications of Coelenterazine H:

Bioluminescence Imaging: Coelenterazine H is commonly used in bioluminescence imaging to monitor cellular and molecular processes in live cells and animals. It reacts with luciferase enzymes to produce light, allowing researchers to visualize and quantify biological activities in real-time. This technique is invaluable in studying disease progression, drug efficacy, and gene expression in vivo.

Reporter Gene Assays: In molecular biology, Coelenterazine H serves as a substrate in reporter gene assays to measure the activity of specific genes or promoters. When used with luciferase reporter systems, it provides a sensitive and quantitative method for tracking gene expression levels. These assays are essential for gene regulation studies, screening of potential therapeutic compounds, and functional genomics.

Calcium Imaging: Coelenterazine H can be employed in calcium imaging experiments to study intracellular calcium dynamics. It is used in conjunction with aequorin, a calcium-sensitive photoprotein, to detect changes in calcium concentration by emitting bioluminescence. This application is crucial for understanding calcium signaling pathways and their role in various physiological and pathological conditions.