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Calcium Green 1 AM | 186501-28-0
| Catalog Number | A14-0033 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C59H53Cl2N3O26 |
| Molecular Weight | 1291.0 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0033 | -- | $-- |
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Product Introduction
Calcium Green 1 AM is a cell-permeant acetyloxy-methyl ester of Calcium Green 1, a fluorescent calcium indicator. Calcium Green 1 is a cell-impermeant fluorescent calcium indicator that is suitable for detecting low levels of calcium such as in resting cells.
Chemical Information |
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|---|---|
| Synonyms | N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[2-[2-[5-[[[3',6'-bis(acetyloxy)-2',7'-dichloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]-glycine, (acetyloxy)methyl ester |
| Purity | ≥90% |
| Canonical SMILES | CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=C(C=C(C=C1)NC(=O)C2=CC3=C(C=C2)C4(C5=CC(=C(C=C5OC6=CC(=C(C=C64)Cl)OC(=O)C)OC(=O)C)Cl)OC3=O)OCCOC7=CC=CC=C7N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChI | InChI=1S/C59H53Cl2N3O26/c1-31(65)79-27-83-53(71)23-63(24-54(72)84-28-80-32(2)66)45-9-7-8-10-47(45)77-15-16-78-52-18-38(12-14-46(52)64(25-55(73)85-29-81-33(3)67)26-56(74)86-30-82-34(4)68)62-57(75)37-11-13-40-39(17-37)58(76)90-59(40)41-19-43(60)50(87-35(5)69)21-48(41)89-49-22-51(88-36(6)70)44(61)20-42(49)59/h7-14,17-22H,15-16,23-30H2,1-6H3,(H,62,75) |
| InChI Key | NXJYZXBURMGUMF-UHFFFAOYSA-N |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 506 nm |
| Emission | 531 nm |
| Storage | Store at -20°C |
Calcium Green 1 AM is a synthesized fluorescent calcium indicator specifically designed for cellular applications to detect calcium ion concentrations. Unlike its precursor, Calcium Green 1, which is cell-impermeant, Calcium Green 1 AM is modified with an acetyloxy-methyl ester linkage, allowing it to permeate cell membranes effortlessly. Once inside the cell, cellular esterases cleave the ester linkage, releasing Calcium Green 1 into the intracellular environment where it selectively binds to calcium ions. This binding results in increased fluorescence intensity, providing a measurable indication of calcium concentration inside a cell. Its high sensitivity to calcium and cell-permeant nature make it a preferred choice for researchers studying cellular calcium dynamics.
One critical application of Calcium Green 1 AM is in neurobiology for monitoring neuronal activity. Calcium ions play a crucial role in synaptic transmission and neuronal firing, and Calcium Green 1 AM can help visualize these rapid changes in real-time. By loading neurons with the indicator, researchers can image calcium influx associated with synaptic activity, thereby gaining insights into neuronal communication and function. This application is especially valuable for studying neural networks, synaptic plasticity, and the neuronal responses to various pharmacological agents.
Another essential use of Calcium Green 1 AM is in cardiomyocyte research to investigate heart muscle function. Calcium ions are pivotal for cardiac contractility, and any dysregulation can lead to pathological conditions. Using this dye, researchers can assess calcium transient dynamics in cardiac cells, monitoring how calcium levels rise and fall in response to electrical stimulation. This is crucial for understanding the excitation-contraction coupling in healthy and diseased states, and for evaluating the effects of drugs that modulate cardiac calcium handling.
Calcium Green 1 AM is also employed in cancer research to study the role of calcium signaling in cell proliferation and apoptosis. Aberrant calcium signaling can lead to uncontrolled cell division or trigger cell death pathways. By using this indicator, researchers can measure basal calcium levels and transient changes in response to various treatments, helping to unravel the complex landscape of cancer cell signaling and identify potential therapeutic targets.
Lastly, Calcium Green 1 AM is used in plant sciences to study calcium’s role in plant cell signaling and physiology. Calcium signals in plants are pivotal during processes like stomatal closure, response to pathogens, and adaptation to environmental stresses. The dye allows researchers to visualize these signals in real-time, contributing to understanding the molecular mechanisms plants employ to respond to their environment and to develop strategies for improving crop resistance and performance under stress conditions.
Applications of Fluorescent Probes & Dyes
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