Coelenterazine 400a | 70217-82-2

Coelenterazine 400a, a luminescent substrate renowned for its applications in bioluminescence studies and biological assays, offers a plethora of uses. Below are four key applications of Coelenterazine 400a:

Bioluminescence Imaging: Illuminating the realms of live cell and animal imaging, Coelenterazine 400a emerges as a quintessential tool for delving into gene expression, protein-protein interactions, and cellular functions in real-time. Upon entering cells housing luciferase enzymes, it triggers vibrant bioluminescent signals that unveil biological processes with vivid clarity, enabling quantitative analysis.

Reporter Gene Assays: Serving as the cornerstone for reporter gene assays, Coelenterazine 400a steps into the realm of scrutinizing the activity of specific promoters or DNA regulatory elements. The bioluminescent signals emanating in the presence of luciferase tags serve as guiding lights, illuminating transcriptional activities and aiding in the nuanced assessment of gene regulation intricacies. This application stands as a linchpin for untangling the elaborate patterns of gene expression and sifting through potential drug candidates with a discerning eye for detail.

Calcium Signaling Studies: Spearheading the frontier of calcium signaling research, Coelenterazine 400a assumes a pivotal role in quantifying intracellular calcium levels. Collaborating with aequorin-based calcium indicators, it radiates light upon binding to calcium ions, offering a visual depiction of calcium fluxes within the cellular milieu. This functionality acts as a foundational pillar for exploring diverse physiological processes, such as muscle contraction, neurotransmission, and cell proliferation, shedding luminous insights on the complexities of cellular orchestration.

Pathogen Detection: Embracing the challenge of detecting pathogens, Coelenterazine 400a takes the spotlight in diagnostic assays aimed at pinpointing pathogenic microorganisms. Through its amalgamation with specific luciferase enzymes, a bioluminescent reaction unfurls in the presence of the target pathogen, culminating in a detectable signal. This method marries heightened sensitivity and specificity, rendering it a potent tool for swiftly identifying microbial invaders in clinical and environmental samples.