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BDY FL, SE | 146616-66-2
| Catalog Number | R01-0009 |
| Category | NHS Esters |
| Molecular Formula | C18H18BF2N3O4 |
| Molecular Weight | 389.166 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R01-0009 | 50 mg | $519 |
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Product Introduction
BODIPY dyes are used to generate fluorescent conjugates of proteins, nucleotides, oligonucleotides and dextrans, as well as to prepare fluorescent enzyme substrates, fatty acids, phospholipids, lipopolysaccharides, receptor ligands and polystyrene microspheres.
Chemical Information |
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|---|---|
| Synonyms | 3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5,5-difluoro-7,9-dimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide; 7-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5,5-difluoro-1,3-dimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide; 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid, Succinimidyl Ester; BDP FL NHS ester |
| Purity | NMR 1H, HPLC-MS (95+%) |
| Canonical SMILES | [B-]1(N2C(=CC(=C2C=C3[N+]1=C(C=C3)CCC(=O)ON4C(=O)CCC4=O)C)C)(F)F |
| InChI | InChI=1S/C18H18BF2N3O4/c1-11-9-12(2)22-15(11)10-14-4-3-13(23(14)19(22,20)21)5-8-18(27)28-24-16(25)6-7-17(24)26/h3-4,9-10H,5-8H2,1-2H3 |
| InChI Key | SDVMGNSTOAJONW-UHFFFAOYSA-N |
| Solubility | Good in organic solvents (DMF, DMSO, dichlromethane), limited in water |
| Appearance | orange solid |
- Product Specification
- Application
| ε, L⋅mol-1⋅cm-1 | 92000 |
| Fluorescence Quantum Yield | 0.97 |
| Excitation | 502 nm |
| Emission | 510 nm |
| Storage | Store at -20°C |
BDY FL, SE is a fluorescent dye commonly used in various bioscience applications due to its high sensitivity and specificity. Here are some key applications of BDY FL, SE:
Cell Imaging: BDY FL, SE is widely used in cell imaging to stain and visualize specific structures or proteins within cells. Its high fluorescence intensity allows for detailed imaging of cellular components, such as membranes, organelles, and cytoskeletal elements. This application is essential for studying cell morphology, dynamics, and interactions in both live and fixed cells.
Flow Cytometry: BDY FL, SE is frequently employed in flow cytometry to label cell populations for various analyses. By conjugating BDY FL, SE to antibodies or other markers, cells can be accurately identified and quantified based on fluorescence intensity. This technique is vital for immunophenotyping, cell sorting, and studying cell cycle dynamics.
Molecular Biology: In molecular biology, BDY FL, SE is often used to label nucleic acids for applications such as fluorescence in situ hybridization (FISH) and quantitative PCR. The dye can be covalently attached to DNA or RNA probes, allowing for the detection and quantification of specific sequences. This facilitates the study of gene expression, chromosomal abnormalities, and microbial detection.
Protein Studies: BDY FL, SE can be used to label proteins for various biochemical and biophysical experiments. By conjugating the dye to proteins, researchers can study protein localization, interactions, and function using fluorescence-based techniques. This application is crucial for understanding protein dynamics, pathway analysis, and drug discovery.
Applications of Fluorescent Probes & Dyes
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