Sulfo-Cyanine5 Allyl (ethyl) | CAS 925915-11-3

Sulfo-Cyanine5 Allyl (ethyl) is a water-soluble cyanine dye designed for fluorescent labeling in bioconjugation workflows, offering a long-wavelength emission that is commonly paired with Cy5-compatible imaging and detection channels. Its allyl functionality supports conjugate construction and incorporation into labeling reagents for fluorescence microscopy, flow cytometry, and fluorescence-based assay development, where robust aqueous handling is important for consistent staining and probe preparation.

1. Fluorescent Antibody Labeling

Sulfo-Cyanine5 Allyl (ethyl) is frequently used by antibody and protein labeling teams to generate Cy5-channel conjugates for fluorescence imaging and immunoassays. In typical workflows, the dye is incorporated into antibody conjugates or secondary labeling reagents used to visualize antigen distribution in fixed samples, to track binding in fluorescence readouts, or to build standardized labeling controls for assay development. The sulfonated, water-soluble character helps maintain dye performance in aqueous buffers during conjugate preparation and downstream assay handling.

2. Flow Cytometry Detection Reagents

Sulfo-Cyanine5 Allyl (ethyl) supports the development of Cy5-compatible flow cytometry dyes and staining reagents used to quantify biomolecular binding and cellular labeling signals. Researchers commonly incorporate the dye into labeling constructs for surface marker detection, enabling multicolor panels where a far-red emission channel is advantageous for reducing spectral overlap with shorter-wavelength fluorophores. The dye's water solubility and fluorescence in aqueous staining conditions make it practical for preparing consistent staining reagents for instrument-based analysis.

3. Fluorescence Microscopy Labeling

Sulfo-Cyanine5 Allyl (ethyl) is applied in fluorescence microscopy workflows to produce fluorescent labeling reagents for cellular and biomolecular imaging in the far-red spectral region. Teams developing imaging probes often use the dye to generate conjugates for tracking targets in microscopy experiments, including labeled antibodies, affinity reagents, and other fluorescent biomolecule constructs. The allyl-enabled dye incorporation supports probe construction for imaging studies where reliable aqueous handling and Cy5-channel compatibility are key.

4. Fluorescent Bioassay Development

Sulfo-Cyanine5 Allyl (ethyl) is used in fluorescence assay development to prepare labeled reagents that report binding or interaction events through far-red fluorescence. Assay developers commonly integrate the dye into detection conjugates used in plate-based fluorescence readouts, enabling consistent signal generation in aqueous assay buffers and compatibility with standard fluorescence plate readers configured for Cy5-like excitation/emission settings. This makes the dye useful for building fluorescence standards, assay components, and labeled controls used during assay optimization and method transfer.