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Acridine homodimer | CAS 57576-49-5
| Catalog Number | A19-0036 |
| Category | DNA Stains |
| Molecular Formula | C38H42Cl2N6O2 |
| Molecular Weight | 685.69 |
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Product Introduction
Acridine homodimer is a fluorescent dye known for its intercalating properties within nucleic acid structures, particularly in DNA, where it binds by inserting between base pairs. This compound features a conjugated aromatic system that facilitates its excitation and emission in the visible spectrum, making it useful in fluorescence microscopy and nucleic acid staining applications. Acridine homodimer is often employed in flow cytometry and fluorescence-based assays to visualize and quantify nucleic acid content, leveraging its ability to exhibit increased fluorescence upon binding to double-stranded DNA.
Chemical Information
Application
Chemical Information
| Synonyms | Bis-(6-chloro-2-methoxy-9-acridinyl)spermine |
| SMILES | COC1=CC2=C(C3=C(C=C(C=C3)Cl)N=C2C=C1)NCCCNCCCCNCCCNC4=C5C=C(C=CC5=NC6=C4C=CC(=C6)Cl)OC |
| InChI | InChI=1S/C38H42Cl2N6O2/c1-47-27-9-13-33-31(23-27)37(29-11-7-25(39)21-35(29)45-33)43-19-5-17-41-15-3-4-16-42-18-6-20-44-38-30-12-8-26(40)22-36(30)46-34-14-10-28(48-2)24-32(34)38/h7-14,21-24,41-42H,3-6,15-20H2,1-2H3,(H,43,45)(H,44,46) |
| InChIKey | NMKIHZILXGGHHL-UHFFFAOYSA-N |
Application
Acridine homodimer is a fluorescent dye with a wide range of applications in molecular biology and biomedical research. Here are some key applications of acridine homodimer:
DNA Staining: Acridine homodimer is extensively used for nucleic acid staining in various types of assays, such as gel electrophoresis and fluorescence microscopy. It preferentially binds to double-stranded DNA, allowing for the visualization of genetic material under UV light. This makes it a valuable tool for detecting DNA fragments and assessing DNA integrity and quantity.
Apoptosis Detection: Acridine homodimer is employed in apoptosis detection assays to distinguish between live, necrotic, and apoptotic cells. By using its combination with other dyes like ethidium bromide or propidium iodide, researchers can observe changes in cell membrane integrity during cell death processes. This helps in understanding the mechanisms of cell death and evaluating the efficacy of anti-cancer treatments.
Cell Viability Assays: In cell viability assays, acridine homodimer serves as a fluorescent marker to assess the health and viability of cells. It can penetrate intact cell membranes but not damaged ones, making it possible to distinguish between live and dead cells. This application is crucial in various fields, including toxicology, pharmacology, and cancer research.
RNA Visualization: Acridine homodimer can also be used for RNA visualization in biological samples. While it has a higher affinity for DNA, it can intercalate with RNA, providing a means to observe RNA localization and distribution within cells. This application is particularly useful in studies of gene expression and RNA dynamics.
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