6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt | CAS 238073-40-0

Catalog Number	A14-0121
Category	Calcium, Chloride and Other indicators
Molecular Formula	C20H23K4N3O12
Molecular Weight	653.81

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Product Introduction

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt is a compound that features a complex molecular architecture incorporating a nitrophenyl moiety, which is significant for its potential role in fluorescence quenching applications. As a tetrapotassium salt, it exhibits enhanced solubility in aqueous environments, facilitating its use in biochemical assays and conjugation reactions. The presence of carboxymethyl groups allows for potential interactions with biomolecules, making it suitable for incorporation in various labeling and imaging workflows within a research context.

Chemical Information
Application

Chemical Information

Synonyms	NP-EGTA K Salt; 2-Nitrophenyl-EGTA
SMILES	C1=CC=C(C(=C1)C(COCCOCCN(CC(=O)O)CC(=O)[O-])N(CC(=O)O)CC(=O)[O-])[N+](=O)[O-].[K+].[K+].[K+].[K+]
InChI	InChI=1S/C20H27N3O12.4K/c24-17(25)9-21(10-18(26)27)5-6-34-7-8-35-13-16(22(11-19(28)29)12-20(30)31)14-3-1-2-4-15(14)23(32)33;;;;/h1-4,16H,5-13H2,(H,24,25)(H,26,27)(H,28,29)(H,30,31);;;;/q;4*+1/p-2
InChIKey	UFZDGCQSBBSEHV-UHFFFAOYSA-L
Appearance	Yellow Solid

Application

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt is a nitrophenyl-containing, highly water-soluble tetraaza/polyether scaffold supplied as a potassium salt, making it well suited for aqueous fluorescence assay development where controlled signal modulation and conjugate-ready carboxylate functionality are required. In fluorescence workflows, the nitroaryl motif supports absorption-based quenching behavior when positioned in close proximity to a fluorophore, enabling design of distance-dependent or proximity-controlled signal suppression formats. Researchers commonly incorporate this type of quencher into oligonucleotide, peptide, or polymer conjugates to reduce background fluorescence and improve readout stability in probe-based experiments.

1. FRET Quencher Design

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt is used by fluorescence technology groups to build FRET pairs and proximity-controlled quenching systems for assay development. By installing the quencher into a donor-acceptor architecture (for example, on a linker within a labeled biomolecule or on a polymer backbone), teams can engineer fluorescence suppression that depends on spatial arrangement, which is useful for monitoring binding-induced conformational changes or reporter release events in molecular imaging reagent prototypes.

2. Molecular Beacon Signal Control

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt supports molecular beacon-style designs where a fluorophore is quenched in the unbound state and fluorescence is recovered upon target-driven structural rearrangement. Oligonucleotide probe developers use this quencher to construct beacon conjugates for fluorescence readouts on standard plate readers and fluorescence microscopes, leveraging its aqueous compatibility and carboxylate-bearing scaffold for conjugation to oligo backbones or linkers used in beacon chemistries.

3. Oligonucleotide Probe Conjugation

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt is frequently selected for nucleic-acid labeling workflows that require a quencher positioned on an oligonucleotide or oligo-derived construct. Molecular biology and chemical biology laboratories use it to create quencher-labeled oligos for hybridization-based fluorescence assays and for multiplex-friendly probe panels where background suppression from free dye is a practical concern. The potassium-salt form and polyether/tetraaza framework help maintain solubility in aqueous assay buffers used for nucleic acid analysis.

4. Polymer and Surface Quenching

6,9-Dioxa-3,12-diazatetradecanedioic acid, 3,12-bis(carboxymethyl)-4-(2-nitrophenyl)-, tetrapotassium salt is applied in biomaterials and materials chemistry settings to generate quencher-functional polymers and surfaces for fluorescence signal management. Researchers incorporate the carboxylate-bearing scaffold into polymer matrices or onto functionalized substrates to create localized quenching environments that can be paired with fluorescent reporters for monitoring release, adsorption, or proximity effects in materials characterization experiments. This approach is commonly used in research-grade coatings, sensor prototypes, and fluorescence-based readout platforms where reducing off-target background improves interpretability.