5-Nitro BAPTA | CAS 124251-83-8

Catalog Number	A14-0063
Category	Calcium, Chloride and Other indicators
Molecular Formula	C22H23N3O12
Molecular Weight	521.43

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Product Introduction

5-Nitro BAPTA is a fluorescent probe characterized by its ability to chelate divalent cations, particularly calcium ions, making it an essential tool in calcium imaging studies. This compound features a BAPTA core structure modified with a nitro group, which influences its spectral properties and enhances its photophysical response to changes in ion concentration. 5-Nitro BAPTA is employed in fluorescence microscopy and biosensing applications, where it facilitates the visualization and quantification of intracellular calcium dynamics.

Chemical Information
Application

Chemical Information

Synonyms	2-[2-[2-[2-[bis(carboxymethyl)amino]phenoxy]ethoxy]-N-(carboxymethyl)-4-nitroanilino]acetic acid
SMILES	C1=CC=C(C(=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=CC(=C2)[N+](=O)[O-])N(CC(=O)O)CC(=O)O
InChI	InChI=1S/C22H23N3O12/c26-19(27)10-23(11-20(28)29)15-3-1-2-4-17(15)36-7-8-37-18-9-14(25(34)35)5-6-16(18)24(12-21(30)31)13-22(32)33/h1-6,9H,7-8,10-13H2,(H,26,27)(H,28,29)(H,30,31)(H,32,33)
InChIKey	JRTCJLPFWVGNQV-UHFFFAOYSA-N
Appearance	Solid Powder

Application

5-Nitro BAPTA is a nitro-substituted BAPTA-based fluorescent calcium chelator used in fluorescence-based studies where calcium binding and chelation are required to control or report intracellular Ca2+ levels. Its Ca2+-binding scaffold is commonly leveraged in chemical biology and fluorescence workflows to tune free calcium concentration, support Ca2+-dependent assay design, and enable mechanistic experiments that depend on calcium availability. In practice, researchers incorporate 5-Nitro BAPTA into calcium modulation strategies alongside fluorescence readouts to interpret calcium-driven biological responses.

1. Calcium Chelation Studies

5-Nitro BAPTA is used by cell and molecular biology laboratories to buffer and control free Ca2+ during fluorescence experiments, helping separate calcium-dependent effects from downstream signaling. Chemical biology groups often pair it with Ca2+-sensitive fluorescent reporters or calcium-responsive biosensors to refine experimental conditions, particularly when precise chelation is needed to interpret transient Ca2+ dynamics. This reagent is also frequently used in mechanistic studies of calcium-regulated processes where modulating available Ca2+ is essential for experimental interpretation.

2. Fluorescence Assay Calibration

5-Nitro BAPTA is applied in fluorescence assay development workflows to establish Ca2+-dependent response baselines and to standardize calcium conditions across experimental runs. Assay developers use it to adjust free Ca2+ in buffer systems and reaction mixtures, supporting reproducible fluorescence readouts in Ca2+-sensitive formats such as cell-free signaling assays and biochemical screening campaigns. By enabling controlled calcium levels, it supports more consistent assay design when fluorescence signals are interpreted as calcium-responsive.

3. Calcium Imaging Controls

5-Nitro BAPTA is used as a calcium modulation reagent in microscopy and imaging studies to generate chelation-based controls for Ca2+-imaging experiments. Imaging groups often include it to suppress or reshape Ca2+-dependent fluorescence changes, which helps validate that observed signal variations originate from calcium fluctuations rather than optical artifacts or non-specific effects. This makes it a practical tool for experimental design in fluorescence microscopy workflows that monitor calcium-dependent cellular events.

4. Signal Modulation in Biosensor Workflows

5-Nitro BAPTA is incorporated into fluorescence biosensor development and optimization efforts where calcium availability must be tuned to evaluate sensor behavior under defined Ca2+ regimes. Researchers developing Ca2+-responsive assays use the chelator to modulate calcium concentration in test buffers and to probe how sensor readouts respond to changes in Ca2+ availability. This supports iterative reagent and assay refinement in fluorescence-based sensing platforms used in chemical biology research.