4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one | CAS 1299464-59-7

4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one is a coumarin-based fluorescent scaffold protected with a triisopropylsilyl ether, making it useful in fluorescence labeling workflows where controlled activation or deprotection can be leveraged during probe preparation. In research settings, this type of coumarin chromophore is commonly incorporated into conjugates and imaging reagents that benefit from strong visible fluorescence and compatibility with standard fluorescence microscopy and plate-reader readouts.

1. Fluorescent Labeling Conjugates

4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one is used as a fluorescent building block for constructing labeled biomolecule conjugates, including small-molecule tags and dye-labeled research reagents. Teams developing fluorescent labeling reagents for protein, peptide, or nucleic-acid workflows often choose coumarin scaffolds because they provide robust emission in common blue/UV-excitation setups, while the silyl-protected oxygen functionality supports downstream derivatization strategies during conjugate assembly.

2. Fluorescence Microscopy Probes

4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one supports the development of microscopy imaging probes used to visualize labeled targets in fixed-sample studies and fluorescence imaging experiments. Researchers constructing fluorescent stains and molecular imaging reagents frequently select coumarin dyes for their bright, readily detectable fluorescence under benchtop microscope illumination, enabling localization studies in cell biology, materials microscopy, and biomaterials characterization when the dye is incorporated into an appropriate targeting or affinity handle.

3. Plate-Reader Fluorescence Assays

4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one is applied in fluorescence assay development as a dye component for preparing assay reagents that report binding, reaction progress, or labeling events through fluorescence intensity. In screening and method-development workflows, coumarin-based reagents are frequently formatted for multiwell plate measurements, where consistent excitation/emission behavior supports routine assay readouts for chemical biology and materials chemistry studies.

4. Polymer And Surface Fluorophore

4-(trifluoromethyl)-7-(triisopropylsilyloxy)-2H-chromen-2-one is also used to introduce coumarin fluorescence into polymer and surface-functionalized materials, supporting fluorescent tagging of coatings, membranes, and biomaterial surfaces. Materials researchers incorporate dye-bearing motifs into functional layers to enable visualization of surface modification, coating uniformity, and transport or adsorption phenomena using standard fluorescence imaging instrumentation.