3,3'-Dipropyloxacarbocyanine iodide | CAS 53213-79-9

3,3’-Dipropyloxacarbocyanine iodide, also known as DiOC6(3), is a fluorescent dye with significant applications in biomedical research. Here are four key applications of this dye:

Mitochondrial Membrane Potential Measurement: Widely utilized for assessing mitochondrial membrane potential in live cells, DiOC6(3) plays a critical role in evaluating the vitality and functionality of mitochondria through fluorescence intensity analysis. This data is pivotal for exploring cellular bioenergetics and unraveling the intricacies of diseases associated with mitochondrial abnormalities.

Cell Viability Assays: In the realm of cell viability assessments, DiOC6(3) stands out as a versatile tool when combined with other dyes to gauge cell survival rates. By contrasting fluorescence signals from live and deceased cells, researchers can quantitatively analyze cell viability under diverse experimental conditions, particularly beneficial for drug screening endeavors and toxicity investigations.

Cellular Membrane Studies: Embodying lipophilic characteristics, DiOC6(3) serves as a valuable dye for labeling various cellular membranes, facilitating the visualization of cellular structures via fluorescence microscopy. This capability empowers scientists to delve into membrane dynamics, organization, and compartmentalization within cells, offering profound insights into intracellular trafficking mechanisms and membrane-related processes.

Flow Cytometry: Integrating seamlessly into flow cytometry analyses, DiOC6(3) plays a pivotal role in scrutinizing membrane potential fluctuations across a large cell population. Through quantifying fluorescence intensity variations, researchers gain a comprehensive understanding of mitochondrial function heterogeneity within cell samples, an indispensable tool for investigating apoptosis and cellular stress responses comprehensively.