Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore

June 9th, 2026 10:00 AM EDT

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3,3'-Diheptyloxacarbocyanine iodide

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3,3'-Diheptyloxacarbocyanine iodide

3,3'-Diheptyloxacarbocyanine iodide | CAS 53213-83-5

Catalog Number	A16-0165
Category	Cell membrane Fluorescent Probes
Molecular Formula	C31H41IN2O2
Molecular Weight	600.57

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Product Introduction

3,3'-Diheptyloxacarbocyanine iodide is a cyanine dye that contains a conjugated aromatic system, enabling it to exhibit distinct absorption and emission properties suitable for fluorescence applications. This compound is often utilized as a membrane probe due to its ability to integrate into lipid bilayers, allowing for the visualization of membrane dynamics in fluorescence microscopy. The dye's spectral behavior and chemical structure support its role in fluorescence imaging workflows, where it functions as a versatile tool for studying cellular processes.

Chemical Information
Product Specification
Application

Chemical Information

Related CAS	79953-80-3 (free base)
Synonyms	DiOC7(3)
SMILES	CCCCCCCN1C2=CC=CC=C2OC1=CC=CC3=[N+](C4=CC=CC=C4O3)CCCCCCC.[I-]
InChI	InChI=1S/C31H41N2O2.HI/c1-3-5-7-9-15-24-32-26-18-11-13-20-28(26)34-30(32)22-17-23-31-33(25-16-10-8-6-4-2)27-19-12-14-21-29(27)35-31;/h11-14,17-23H,3-10,15-16,24-25H2,1-2H3;1H/q+1;/p-1
InChIKey	ANJZLSIIEGUCQL-UHFFFAOYSA-M

Product Specification

Excitation	426
Emission	535

Application

3,3’-Diheptyloxacarbocyanine iodide, also known as DiOC7(3), is a versatile fluorescent dye widely utilized in bioscience research. Here are four key applications of DiOC7(3):

Cell Membrane Potential Measurement: In the realm of cellular physiology, DiOC7(3) emerges as a prominent tool for quantifying alterations in membrane potential across both prokaryotic and eukaryotic cells. By embedding itself within cell membranes, this dye exhibits fluorescence modulation in response to shifts in membrane potential, offering insights into cellular processes tied to ion channel activity, signal transduction, and cellular respiration.

Intracellular Organelles Staining: Delving into the intricate world of cell biology, DiOC7(3) finds purpose in staining intracellular organelles like mitochondria and endoplasmic reticulum. Its affinity for membranes enables vivid visualization of these organelles under the fluorescence microscope, shedding light on organelle dynamics, morphology, and functional roles in diverse cellular pathways.

Flow Cytometry: Embracing the realm of cutting-edge technology, DiOC7(3) plays a pivotal role in flow cytometry assays aimed at evaluating cell viability, apoptosis, and mitochondrial membrane potential. By leveraging this dye to label cells, researchers can swiftly and quantitatively assess the well-being and functional status of cell populations, offering valuable insights for toxicology assessments, drug screening endeavors, and cellular functionality investigations.

Live Cell Imaging: Empowering real-time exploration of dynamic cellular processes, DiOC7(3) stands out as a preferred choice for live cell imaging, preserving cell viability while providing vibrant staining. This capability facilitates continuous monitoring of biological phenomena such as cell division, migration, and interactions, enabling high-resolution imaging and time-lapse studies crucial for unraveling the intricacies of dynamic cellular events.