3,3'-Diheptyloxacarbocyanine iodide | 53213-83-5

3,3’-Diheptyloxacarbocyanine iodide, also known as DiOC7(3), is a versatile fluorescent dye widely utilized in bioscience research. Here are four key applications of DiOC7(3):

Cell Membrane Potential Measurement: In the realm of cellular physiology, DiOC7(3) emerges as a prominent tool for quantifying alterations in membrane potential across both prokaryotic and eukaryotic cells. By embedding itself within cell membranes, this dye exhibits fluorescence modulation in response to shifts in membrane potential, offering insights into cellular processes tied to ion channel activity, signal transduction, and cellular respiration.

Intracellular Organelles Staining: Delving into the intricate world of cell biology, DiOC7(3) finds purpose in staining intracellular organelles like mitochondria and endoplasmic reticulum. Its affinity for membranes enables vivid visualization of these organelles under the fluorescence microscope, shedding light on organelle dynamics, morphology, and functional roles in diverse cellular pathways.

Flow Cytometry: Embracing the realm of cutting-edge technology, DiOC7(3) plays a pivotal role in flow cytometry assays aimed at evaluating cell viability, apoptosis, and mitochondrial membrane potential. By leveraging this dye to label cells, researchers can swiftly and quantitatively assess the well-being and functional status of cell populations, offering valuable insights for toxicology assessments, drug screening endeavors, and cellular functionality investigations.

Live Cell Imaging: Empowering real-time exploration of dynamic cellular processes, DiOC7(3) stands out as a preferred choice for live cell imaging, preserving cell viability while providing vibrant staining. This capability facilitates continuous monitoring of biological phenomena such as cell division, migration, and interactions, enabling high-resolution imaging and time-lapse studies crucial for unraveling the intricacies of dynamic cellular events.