Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate | CAS 80969-52-4

Catalog Number	A16-0145
Category	Mitochondrial Fluorescent Probes
Molecular Formula	C23H26N2O3S
Molecular Weight	410.532

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Product Introduction

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate is a cationic styryl dye known for its extended conjugated π-electron system, which facilitates strong absorption and emission properties in the visible spectrum. This dye is often utilized in fluorescence microscopy and bioimaging applications, where it functions as a membrane probe due to its affinity for lipid bilayers. Its structural design includes a dimethylamino group that enhances charge transfer interactions, making it suitable for investigating membrane dynamics and cellular environments.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	4-Di-1-ASP, TS
SMILES	CC1=CC=C(C=C1)S(=O)(=O)[O-].C[N+]1=CC=C(C=C1)C=CC2=CC=C(C=C2)N(C)C
InChI	InChI=1S/C16H19N2.C7H8O3S/c1-17(2)16-8-6-14(7-9-16)4-5-15-10-12-18(3)13-11-15;1-6-2-4-7(5-3-6)11(8,9)10/h4-13H,1-3H3;2-5H,1H3,(H,8,9,10)/q+1;/p-1
InChIKey	XQNXLVUPJKQLPU-UHFFFAOYSA-M

Product Specification

Excitation	271 nm
Emission	475 nm

Application

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate is a cyanine-family fluorescent dye used as a bright, charge-stabilized molecular stain for fluorescence-based localization and uptake studies. Its conjugated, styryl-pyridinium framework supports strong visible emission, while the pyridinium salt form improves handling in aqueous labeling workflows. Researchers commonly employ this dye when a membrane-associated or polarity-sensitive fluorescent signal is needed for microscopy and quantitative fluorescence readouts.

1. Fluorescence Microscopy Staining

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate is used in fluorescence microscopy to visualize stained cellular compartments and membrane-associated distributions in fixed samples and imaging experiments. In practice, investigators incorporate the dye into staining protocols to generate high-contrast fluorescence images for qualitative localization studies, including time-resolved or comparative imaging across experimental conditions. The dye's strong visible emission and cationic, conjugated structure make it a common choice for building imaging workflows where robust signal is required without complex probe engineering.

2. Flow Cytometry Fluorescent Labeling

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate supports flow cytometry workflows that rely on fluorescent intensity readout to quantify stained cell populations. Researchers use the dye as a fluorescent label in assays where relative fluorescence intensity is used to compare uptake, staining level, or membrane-associated signal across samples. Its salt-form presentation and compatibility with standard fluorescence instrumentation make it practical for high-throughput screening of labeled cells, including optimization of staining conditions and gating strategies for downstream analysis.

3. Membrane-Associated Fluorescence Assays

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate is frequently applied in plate-based fluorescence assays designed to report on membrane-related fluorescence changes. In these workflows, the dye is used as a staining reagent to generate a measurable fluorescence response that can be tracked under different buffer compositions, treatment conditions, or experimental perturbations. This makes the dye useful for developing comparative fluorescence assays where the readout depends on the dye's interaction with cellular membranes or membrane-like environments, enabling convenient monitoring with standard fluorescence plate readers.

4. Fluorescent Standards And Imaging Controls

Trans-4-[4-(Dimethylamino)styryl]-1-methylpyridinium p-toluenesulfonate is also used as a reference fluorophore for instrument setup and assay controls in fluorescence imaging and detection experiments. Laboratories incorporate the dye into control preparations to verify excitation/emission alignment, support channel assignment, and maintain consistency across runs when building microscopy or cytometry methods. Because it is a defined fluorescent dye rather than a target-responsive probe, it is particularly useful for method development and routine quality checks where stable fluorescence behavior and straightforward handling are valued.