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Tetramethylrhodamine isothiocyanate (mixed isomers) | CAS 95197-95-8
| Catalog Number | A16-0019 |
| Category | Mitochondrial Fluorescent Probes |
| Molecular Formula | C25H21N3O3S |
| Molecular Weight | 443.5 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Tetramethylrhodamine isothiocyanate (TRITC) is an amine-reactive probe for labeling proteins and nucleic acids. It is composed of a base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group.
Chemical Information
Product Specification
Application
| Synonyms | 5(6)-Tetramethylrhodamine isothiocyanate; 5(6)-TRITC; TRITC; 9-(2-carboxyisothiocyanatophenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt |
| Purity | ≥95% |
| Appearance | Solid Powder |
| Excitation | 552 nm |
| Emission | 575 nm |
| Storage | Store at -20°C |
Tetramethylrhodamine isothiocyanate, a versatile fluorescent dye widely employed in bioscience, serves a multitude of purposes. Dive into four key applications of this dynamic dye:
Immunofluorescence: A fundamental technique in immunofluorescence, Tetramethylrhodamine isothiocyanate emerges as the premier choice for labeling antibodies. Its intricate attachment enables the visualization of specific proteins within cells or tissue specimens under a fluorescence microscope, producing sharp, high-resolution imagery that precisely pinpoints the location of target molecules within biological structures, revealing the intricate details of cellular processes.
Flow Cytometry: In the complex world of flow cytometry, Tetramethylrhodamine isothiocyanate takes the spotlight in cell sorting and analysis. Cells labeled with this vibrant fluorescent dye undergo thorough assessments or fluorescence-based sorting, essential for isolating specific cell subpopulations in both research and clinical diagnostics, enhancing our understanding of cell dynamics to a remarkably detailed level.
Fluorescence Microscopy: The versatility of this dye shines in fluorescence microscopy, playing a crucial role in labeling and tracking biomolecules within live cells. The vivid and enduring fluorescence of Tetramethylrhodamine isothiocyanate allows for prolonged observation of dynamic processes, such as protein transport, cell signaling, and mitosis. Seamlessly integrated into live-cell imaging technologies, it emerges as a versatile ally in unraveling the intricate ballet of cellular biology.
Protein Labeling: In the realm of protein labeling, Tetramethylrhodamine isothiocyanate stands out as a powerful tool for various biochemical and biophysical investigations. By pairing this fluorescent probe with proteins, researchers unlock pathways for techniques like fluorescence resonance energy transfer (FRET), enabling the examination of protein interactions and structural dynamics at a molecular level. This deep dive into protein function and dynamics reveals new layers of insight, illuminating the inner workings of vital biological processes.
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