Rhodamine-123 | CAS 62669-70-9

Catalog Number	A16-0170
Category	Mitochondrial Fluorescent Probes
Molecular Formula	C21H17ClN2O3
Molecular Weight	380.82

Catalog Number	Size	Price	Quantity
A16-0170	50 mg	$199
A16-0170	250 mg	$419

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Product Introduction

Rhodamine-123 is a cell-permeant green-fluorescent probe that is taken up by active mitochondria.

Chemical Information
Product Specification
Application

Synonyms	Rh 123
Canonical SMILES	COC(=O)C1=CC=CC=C1C2=C3C=CC(=N)C=C3OC4=C2C=CC(=C4)N.Cl
InChI	InChI=1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChIKey	MYFATKRONKHHQL-UHFFFAOYSA-N
Appearance	Solid Powder

Excitation	507 nm
Emission	529 nm

Rhodamine-123, a fluorescent dye renowned for its specificity and sensitivity, finds widespread utility in diverse scientific domains. Here are four key applications of Rhodamine-123:

Mitochondrial Staining: Renowned for its vibrant fluorescent properties, Rhodamine-123 serves as a staple for staining mitochondria in live cells, enabling meticulous investigations into mitochondrial dynamics and functionality. This dye exhibits a penchant for active mitochondria, unveiling intricate details through high-resolution imaging. Its pivotal role in assessing mitochondrial well-being and probing diseases linked to mitochondrial aberrations underscores its indispensability in cellular research.

Cell Viability Assays: Delving into cellular vitality, Rhodamine-123 emerges as a cornerstone in conducting cell viability assays, shedding light on the metabolic robustness of cells. By discerning variations in fluorescence intensity, scientists unravel the proportion of viable cells within a population—a critical aspect in gauging the cytotoxic impact of pharmaceutical agents and experimental compounds in the realm of pharmacology.

Flow Cytometry: In the realm of flow cytometry, Rhodamine-123 assumes a pivotal role in scrutinizing the mitochondrial membrane potential of cells, offering a window into cellular vitality and metabolic vigor. Its fluorescence yield, quantifiable by state-of-the-art flow cytometers, furnishes researchers with invaluable insights into cellular well-being and metabolic activity. This technique, straddling basic research and clinical diagnostics, plays a pivotal role in unraveling cellular intricacies.

Drug Resistance Studies: Spearheading investigations into drug resistance mechanisms, Rhodamine-123 emerges as a linchpin in scrutinizing the efflux dynamics of cancer cells, unmasking the inner workings of drug resistance pathways. By evaluating the dye efflux patterns, researchers decode the activity of drug transporters like P-glycoprotein, pivotal players in conferring multidrug resistance. This application, pivotal for devising strategies to thwart resistance mechanisms and enhance cancer treatment efficacy, underscores the transformative potential of Rhodamine-123 in oncological research.