Stains All | 7423-31-6

Stains All is a cationic carbocyanine dye primarily employed in biochemistry for the differential staining of nucleic acids and proteins. Its unique ability to bind with both DNA and RNA, as well as various proteins, stems from its positive charge, which facilitates interaction with the negatively charged phosphate groups present in these macromolecules. Stains All is especially useful in gel electrophoresis and other staining protocols where distinct visualization of multiple biomolecules within a single sample is required. This versatility makes it an indispensable tool in the laboratory for identifying and quantifying nucleic acids and proteins in complex mixtures.

One of the key applications of Stains All is in the detection and visualization of nucleic acids during agarose or polyacrylamide gel electrophoresis. The dye selectively binds to the nucleic acids, producing a color change that is easily visible under specific lighting conditions, thus allowing researchers to pinpoint specific bands corresponding to DNA or RNA. This application is crucial for molecular biology experiments that require the precise measurement and manipulation of genetic material, such as cloning, sequencing, and polymerase chain reactions (PCR).

Another significant application of Stains All is in the differentiation of proteins, particularly those containing acidic domains or glycosaminoglycans. The dye can identify calcium-binding proteins and proteoglycans by imparting a unique coloration pattern that is distinguishable from other protein types. This property is particularly valuable in proteomics and the study of cellular processes involving calcium signaling and extracellular matrix interactions, aiding researchers in understanding protein function and disease mechanisms.

Stains All is also used in the analysis of lipopolysaccharides and other complex carbohydrates. By revealing specific staining patterns, this application enhances the study of bacterial endotoxins and other glycoconjugates involved in cell wall structure and immune response. This capability is instrumental in microbiology and immunology research, where understanding the composition and function of these molecules can lead to breakthroughs in vaccine development and antibacterial therapies. Lastly, its utility in staining polyphosphate has made it an essential tool for investigations into energy storage and metabolism.