Rhodamine 700 perchlorate | CAS 63561-42-2

Rhodamine 700 perchlorate is a red-to-near-infrared emitting rhodamine dye supplied as a perchlorate salt, enabling convenient preparation of fluorescent labeling solutions for microscopy and fluorescence-based assays. Its strong absorbance and emission in the far-red region make it a practical fluorophore for multi-color imaging workflows where spectral separation from common green and orange channels is required. The cationic, highly fluorescent nature of rhodamine scaffolds also supports its use as a bright reporter in conjugate and staining formats.

1. Fluorescence Microscopy Staining

Rhodamine 700 perchlorate is used as a far-red fluorescent stain for cellular and biomaterial imaging workflows in which researchers need robust signal in the red/NIR spectral window. Typical users include cell biology laboratories and microscopy core facilities preparing labeled samples for widefield fluorescence microscopy and confocal imaging, often to visualize labeled biomolecules, track surface-associated components, or provide contrast in multi-channel experiments. Its rhodamine dye character supports high-contrast visualization when excitation and emission are matched to standard far-red filter sets and laser lines used in research microscopes.

2. Flow Cytometry Fluorescent Labeling

Rhodamine 700 perchlorate is frequently employed as a fluorescence label or reporter dye in flow cytometry experiments where far-red emission helps reduce spectral overlap with fluorophores in the green/orange range. Researchers use it to generate stained cell populations for fluorescence readouts that benefit from red-shifted detection, including labeling strategies for biomolecule tracking in assay development and screening workflows. In practice, it is leveraged when instrument configurations have compatible excitation sources and far-red collection optics, enabling reliable gating and population analysis based on dye fluorescence intensity.

3. Fluorescence Bioassay Reporter

Rhodamine 700 perchlorate is applied as a fluorescent reporter in fluorescence-based bioassays and reagent development studies that require a far-red emitting dye for plate-based or cuvette-based measurements. Many users in chemical biology and assay development incorporate rhodamine-derived fluorescence into labeling readouts, wash-based detection formats, or conjugate-based assay components where spectral positioning in the red region supports multiplexing. The dye's bright emission supports straightforward quantification of fluorescence intensity as an assay signal, provided the assay design uses excitation/emission conditions aligned with Rhodamine 700's far-red spectral profile.

4. Fluorophore-Conjugate Construction

Rhodamine 700 perchlorate is commonly used as a starting fluorescent building block for preparing rhodamine-based conjugates used in molecular imaging reagent development and fluorescent labeling research. Bioconjugation-focused groups use it to build labeled constructs for tracking biomolecules, generating fluorescent standards, or assembling dye-containing probes for downstream experimental workflows. In these settings, researchers typically select Rhodamine 700 perchlorate when they want a far-red fluorophore core that can be integrated into conjugates compatible with fluorescence imaging and detection instrumentation.