Resorufin benzyl ether | 87687-02-3

Resorufin benzyl ether, a fluorescent probe commonly employed in biochemical and cell-based assays, serves a myriad of purposes. Here are four key applications of resorufin benzyl ether:

Enzyme Activity Assays: In the dynamic realm of enzyme kinetics, resorufin benzyl ether emerges as a prevalent choice for monitoring the activity of specific enzymes like cytochrome P450s. Upon enzymatic hydrolysis, this probe liberates resorufin, which emits a vibrant red fluorescence. This unique property enables the sensitive real-time detection and quantification of enzyme activity, illuminating the intricate workings of enzymatic processes.

Drug Metabolism Studies: Delving into the realm of pharmacology, resorufin benzyl ether finds its niche in elucidating drug metabolism pathways, particularly those involving liver enzymes. By tracking product formation, researchers can gauge the activity levels of metabolizing enzymes and explore the interplay between drugs and these enzymes. This knowledge is pivotal in unraveling drug clearance mechanisms and potential drug-drug interactions.

Cell Viability Assays: In the realm of cell biology, resorufin benzyl ether emerges as a valuable asset in determining the vitality and metabolic status of cells. As cells metabolize this compound, the ensuing fluorescence serves as a beacon, indicating cellular metabolic vigour. This application finds significance in toxicology studies, drug screening endeavors, and the assessment of cytotoxic impacts induced by diverse compounds, shedding light on cellular health and functionality.