Resorufin benzyl ether | CAS 87687-02-3

Catalog Number	A16-0189
Category	Other Cell Fluorescent Probes
Molecular Formula	C19H13NO3
Molecular Weight	303.3

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Product Introduction

Resorufin benzyl ether is a red fluorometric probe that acts as a substrate for cytochrome P450 CYP3A4. It is used to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities.

Chemical Information
Product Specification
Application

Synonyms	Benzyloxyresorufin; 7-Benzyloxyresorufin; 7-(benzyloxy)-3H-phenoxazin-3-one
Purity	≥98%
Appearance	Solid Powder

Excitation	571 nm
Emission	585 nm
Storage	Store at -20°C

Resorufin benzyl ether, a fluorescent probe commonly employed in biochemical and cell-based assays, serves a myriad of purposes. Here are four key applications of resorufin benzyl ether:

Enzyme Activity Assays: In the dynamic realm of enzyme kinetics, resorufin benzyl ether emerges as a prevalent choice for monitoring the activity of specific enzymes like cytochrome P450s. Upon enzymatic hydrolysis, this probe liberates resorufin, which emits a vibrant red fluorescence. This unique property enables the sensitive real-time detection and quantification of enzyme activity, illuminating the intricate workings of enzymatic processes.

Drug Metabolism Studies: Delving into the realm of pharmacology, resorufin benzyl ether finds its niche in elucidating drug metabolism pathways, particularly those involving liver enzymes. By tracking product formation, researchers can gauge the activity levels of metabolizing enzymes and explore the interplay between drugs and these enzymes. This knowledge is pivotal in unraveling drug clearance mechanisms and potential drug-drug interactions.

Cell Viability Assays: In the realm of cell biology, resorufin benzyl ether emerges as a valuable asset in determining the vitality and metabolic status of cells. As cells metabolize this compound, the ensuing fluorescence serves as a beacon, indicating cellular metabolic vigour. This application finds significance in toxicology studies, drug screening endeavors, and the assessment of cytotoxic impacts induced by diverse compounds, shedding light on cellular health and functionality.