Fluorescein-5-thiosemicarbazide | 76863-28-0

Fluorescein-5-thiosemicarbazide (FTSC) is a versatile fluorescent labeling reagent widely utilized in biochemical and cellular assays. Explore four key applications of FTSC:

Protein Labeling: Highly favored in the scientific realm for protein labeling, FTSC emerges as a prominent fluorescent tag, aiding in the detection and visualization of proteins across various analytical techniques. This strategic labeling technique empowers researchers to precisely trace the localization and interactions of proteins within cells using fluorescence microscopy. Moreover, quantifying labeled proteins in assays like flow cytometry and Western blotting enhances the depth of protein analysis, enriching protein characterization.

DNA and RNA Labeling: FTSC proves invaluable in labeling nucleic acids, enabling researchers to visually track DNA and RNA molecules during experimental procedures. This application holds particular significance in techniques such as in situ hybridization, where labeled nucleic acid probes selectively bind to specific sequences in chromosomes or tissue sections. The emitted fluorescence signal offers insights into gene expression patterns and the spatial arrangement of genetic material, unveiling intricate molecular processes.

Enzyme Assays: In the realm of enzyme assays, FTSC plays a pivotal role in synthesizing fluorescent substrates that allow real-time monitoring of enzymatic activity. Monitoring changes in fluorescence intensity enables researchers to delve into enzyme kinetics, evaluate inhibitor efficacy, and scrutinize the impact of varying conditions on enzyme performance. This pivotal information drives progress in basic research, refines drug development processes, and fosters innovation in enzymology.

Cell Viability and Proliferation Studies: Leveraging the adaptability of fluorescein-labeled molecules, researchers seamlessly incorporate FTSC-labeled reagents into living cells to assess cell viability and proliferation dynamics. These labeled reagents facilitate the staining of live cells, enabling researchers to monitor cell health and division patterns using techniques such as fluorescent microscopy or flow cytometry. This application is essential for toxicity screening, cancer research endeavors, and the exploration of cell cycle dynamics, contributing significantly to advancements in cellular biology and drug discovery initiatives.