Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore

June 9th, 2026 10:00 AM EDT

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4-(4-(dimethylamino)styryl)-n-methylpyridinium iodide

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4-(4-(dimethylamino)styryl)-n-methylpyridinium iodide

4-(4-(dimethylamino)styryl)-n-methylpyridinium iodide | CAS 959-81-9

Catalog Number	A16-0152
Category	Other Cell Fluorescent Probes
Molecular Formula	C16H19IN2
Molecular Weight	366.246

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Product Introduction

4-(4-(Dimethylamino)styryl)-N-methylpyridinium iodide is a fluorescent dye belonging to the styryl dye class, characterized by its conjugated aromatic system that facilitates intense fluorescence. This compound exhibits distinct excitation and emission properties, making it suitable for applications in fluorescence microscopy and cellular imaging workflows. The dye's structure allows it to function as a membrane probe, enabling the visualization of cellular components and supporting research in bioimaging and molecular tracking.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	4-Di-1-ASP
SMILES	C[N+]1=CC=C(C=C1)C=CC2=CC=C(C=C2)N(C)C.[I-]
InChI	InChI=1S/C16H19N2.HI/c1-17(2)16-8-6-14(7-9-16)4-5-15-10-12-18(3)13-11-15;/h4-13H,1-3H3;1H/q+1;/p-1
InChIKey	UJNFDSOJKNOBIA-UHFFFAOYSA-M
Appearance	Solid Powder

Product Specification

Excitation	340
Emission	488

Application

4-(4-(Dimethylamino)styryl)-N-methylpyridinium iodide, also known as DASPMI, is a fluorescent dye widely used in scientific fields. Here are four key applications of DASPMI:

Fluorescence Microscopy: DASPMI, a fluorescent dye utilized in fluorescence microscopy, serves as a crucial stain for various cellular components. Its vibrant fluorescence properties enable researchers to delve into cellular structures and dynamics with exceptional resolution. This indispensable tool facilitates the in-depth exploration of cell morphology and intracellular processes in both live and fixed cells, enriching our understanding of cellular intricacies.

Mitochondrial Imaging: Due to its positive charge and lipophilicity, DASPMI exhibits selective accumulation in mitochondria, making it a premier dye for imaging and evaluating mitochondrial function and vitality. Researchers harness DASPMI to investigate mitochondrial distribution, membrane potential, and alterations linked to metabolic states or mitochondrial disorders. This application empowers the study of mitochondrial dynamics and health, shedding light on crucial aspects of cellular metabolism.

Flow Cytometry: In the realm of flow cytometry, DASPMI plays a pivotal role in analyzing cell populations based on their distinct fluorescence characteristics. By staining cells with DASPMI, researchers can quantitatively assess and segregate cells exhibiting specific mitochondrial properties. This versatile tool finds particular utility in studies concerning cell metabolism, apoptosis, and cancer research, offering valuable insights into cellular behaviors and functions.

Environmental Monitoring: DASPMI finds application in environmental science for the detection and surveillance of microorganisms in water and soil samples. Its ability to stain bacterial cells makes it a valuable asset for evaluating microbial contamination levels and deciphering microbial ecology dynamics. This innovative application aids in environmental health assessments and the formulation of effective strategies for environmental remediation.