TAMRA-PEG4-t-butyl ester | CAS 2353409-64-8

TAMRA-PEG4-t-butyl ester is a TAMRA-based fluorescent labeling reagent featuring a PEG4 spacer and a t-butyl ester that is designed for downstream conjugation workflows. In typical use, the ester functionality enables controlled incorporation into biomolecule or material constructs, while the TAMRA fluorophore provides strong red/orange fluorescence for imaging, labeling, and fluorescence-based assay development.

1. Biomolecule Fluorescent Labeling

TAMRA-PEG4-t-butyl ester is used by chemical biology and bioconjugation teams to prepare fluorescently tagged biomolecules for microscopy and quantitative fluorescence workflows. Researchers commonly incorporate the PEG spacer to improve solubility and reduce steric effects when attaching TAMRA to peptides, proteins, or other labelling targets, supporting clearer visualization in labeling experiments and reagent characterization. The reagent format is also frequently selected when a protected carboxyl functionality is needed during conjugate construction, enabling the TAMRA label to be introduced into larger conjugates used for binding studies, tracking experiments, and fluorescence readouts.

2. Fluorescent Probe Construction

TAMRA-PEG4-t-butyl ester supports fluorescent probe development where a TAMRA reporting group is required as part of a larger sensing or reporting construct. In reagent development laboratories, the PEG4-tethered TAMRA scaffold is incorporated into probe architectures for fluorescence imaging assays, including labeling-based probe formats used to monitor biomolecular interactions or to generate fluorescent standards for assay optimization. The red-emitting TAMRA signal is particularly useful when designing multi-reagent fluorescence experiments that require spectral separation from common green fluorophores.

3. Surface And Polymer Functionalization

TAMRA-PEG4-t-butyl ester is applied in materials research to introduce a fluorescent tag onto polymeric or surface-associated platforms, enabling visualization of functionalized materials and tracking of surface modifications. Biomaterials groups use the PEG spacer to help maintain accessibility of the fluorophore relative to the underlying surface or polymer backbone, which can improve interpretability of fluorescence microscopy or plate-based fluorescence measurements used to confirm functionalization. This approach is frequently used in workflows where fluorescent labeling of coatings, linkers, or conjugate-bearing materials is needed for process monitoring and characterization.

4. Fluorescence Imaging And Tracking

TAMRA-PEG4-t-butyl ester is used as a fluorescent labeling input for imaging and tracking experiments in cell biology and analytical microscopy workflows. Researchers employ TAMRA fluorescence to follow labeled constructs during incubation, wash steps, and localization studies, leveraging the PEG4 spacer to support consistent labeling behavior in heterogeneous experimental conditions. The reagent is also used to generate fluorescent reference materials or labeled components for method development in fluorescence microscopy and fluorescence-based quantification assays.